MSC Flashcards
What is MSC?
Mesenchymal Stem cell. Bone marrow derived stem cells: multipotent.
Where is MSC isolated from?
The bone marrow. 0.0001% of nucleated cells in the BM
What are the 5 steps to isolating MSC?
1) BM scooped out/aspirated and sides scraped to disrupt and remove cells from all microniches 2)marrow sample fractionated on density gradient, this purifies nucleated cells 3)mononuclear fraction plated at low density 4)recovered over 3 week period 5) detatched, subcultured experimented etc
What is a pericyte?
MSCs found throughout fetal and adult tissues are members of the pericyte family of cells ‘advential reticular cells’
Are MSCs mortal?
Oui
Are MSCs telomerase positive or negative?
negative
How can MSC proliferation be enhanced?
low density plating and enrichment
How does MSC differentiation occur in vivo animal models?
- transplantation into mouse models (bone, cartilage, lung engraftment and functional incorportation) - in utero sheep transplantation (cartilage, fat, muscle, heart, BM, cell persistance up to 13 months)
Are MSCs multipotent?
minimally
What are MSCs characterised by?
expression of CD molecules - CD44, CD105, CD45
What are OSIRIS therapeutics?
The leading proponent of MSC-based cell therapy
What are MSC’s immunosuppressive effects?
1 - inhibits T cell responses
What are advantages to MSCs?
easy to derive, easy to culture, easy to differentiate, some species pluripotent, already in the market place, immunosuppressive
What are disadvantages to MSCs?
Limited proliferation (mortal), culture conditions not optimised, severe discomfort during removal, age related decline in number and potentiality, no deffo evidence to show in vivo MSC exist AND have not yet been demonstrated to be a true stem cell¬
Horses?
Tendon therapy - BM taken from sternum of injured horse, isolate MSCs, resuspend MSCs in BM supernatent, implant into core lesion of tendon or ligament, healed horse.
