Tree Of Life Flashcards
Yeast Cell (Eukarya)
-size 8um in diameter
-some Ekarya cells are 500 um in diameter
-virus cell of Escherichia Coli (Bacteria) ~1x3 um
-1000nm = 1um in diameter
Bacteria
-Bacteria can range in size very dramatically
-most are close to 1um, but surgeonfish gut symbiont Epulopiscium (clostridia -> spores) is 600 um x 5um
Subcellular Organizations
ALL cells have
1. Cytoplasmic membrane
2. Cytoplasm containing ribosomes and metabolic enzymes
3. DNA
-some cells have walls: Bacteria, Fungi, Plants
-Animal cells have a cytoskeleton
Prokaryotic Cells
-no organelles
-no membrane-bound nucleus
Eukaryotic Cells
-internal membrane-bound organelles
-DNA within the membrane-bound nucleus
Arrangement of DNA in a Prokaryotic Cell
- DNA condensed with a “nucleoid” region
- Generally a singular circular chromosome
- Haploid
- No sexual reproduction
- Some small circular extra-chromosomal DNA “plasmid”
Arrangement of DNA in a Eukaryotic Cell
- DNA within the nucleus
- Multiple linear chromosomes
- Diploid
- Divide by mitosis
- Produce haploid gametes be meiosis
- Sexual reproduction
Viruses
-No cytoplasm, ribosome, or metabolic activity
-Not alive
Only have: 1. Nucleic acid genome (DNA or RNA)
2. Protein capsid
3. Sometimes a membrane
-must infect a prokaryotic or eukaryotic cell in order to reproduce
Classifying Life Forms
-the classical methods of classifying life forms were based on organism structure and to some degree cell structure
Early Attempts at Classisfication
Linnaeus (1753) 2 kingdoms
-Animalia (higher animals and protozoa)
-Plantae (higher plants, algae and fungi)
-no prokaryotes!
Haeckel (1865) 3 Kingdoms
-Animalia (higher animals)
-Plantae (higher plants and multicellular algae)
-Protista (all microorganisms including bacteria, protozoa, algae, molds, and yeast)
-Haeckal was a believer in Darwin’s theories of evolution and tired to present his classifications in a evolutionary tree!
What word did Haeckal invent and what does it mean?
Phylogeny = the evolutionary history of organisms
Whittaker (1969)
-Five kingdom classification of living organisms
-Monera then Protista then Plantae, Fungi, and Animalia branch off of Protista
Molecular-based approach to Phylogeny
-Carl Woese (1970s)
-16s rRNA in prokaryotes (18s rRNA in eukaryotes)
-ancient molecule that ALL cells have (part of the ribosome, essential for translation)
-changed very slowly over evolutionary time
-sequenced easily
Steps of Molecular-based approach to phylogeny
- Isolate DNA from each organism
- Make copies of rRNA gene by PCR
- Sequence DNA
- Analyze sequence
- Generate phylogenic tree