Transport Flashcards
Active transport
ATP, against conc gradient, selective process
Involve carrier proteins/pumps
How is the cell adapted for active transport
Microvilli, LSA, more carrier proteins, mitochondria for ATP synthesis
Carrier proteins span plasma membrane, bind to molecule or ion to be transported on one side
Describe the process of active transport
Molecule/ion binds to receptor sites on protein
On the inside of the cell/organelle, ATP binds to protein, splitting into ADP, phosphate
Protein molecule change shape, opens to opposite side of membrane
Molecule/ion released to other side of membrane
Phosphate released from protein, causes protein to revert to original shape
Phosphate combines with ADP to ATP
Simple diffusion
All particles are constantly in motion, KE
Random motion, particles constantly bouncing around
Net movement of molecules or ions from a high to low conc until equilibrium formed
Small, non polar molecules easily diffuse
Facilitated diffusion, what doesn’t easily pass through the phospholipid bilayer?
Charged ions, polar molecules do not diffuse easily due to hydrophobic nature of fatty acid tails
Describe the process of facilitated diffusion
Relies on KE of diffusing molecules, down conc gradient
Only occurs at specific points on membrane, protein channels, carrier proteins
How to increase facilitated diffusion rate
Protein channels and carrier proteins can be added to the phospholipid bilayer
How do protein channels work in facilitated diffusion?
Form water filled hydrophilic channels across the membrane
Allow water soluble ions through
Selective channels, gated, open in the presence of a specific ion
Ion binds with the protein causing it to change shape in a way that closes it to one side of the membrane and opens it to the other side
How do carrier proteins work in facilitated diffusion
Span plasma membrane
When a specific molecule is present, binds with a protein
Causes a shape change, molecule released to inside of membrane
Osmosis
The passage of water from a region of high water potential to an area of lower water potential through a selectively permeable membrane
Water potential
Tendency of water molecules in a system to move
Psi, measured in kPa
Pure water=0kPa, highest value
Solutions have -ve value
Cells in higher water potential than cell solution in plant cells
Water diffuses in through partially permeable membrane
Contents expand, push out on wall
Wall pushes back on cell contents
Force of wall pushing on cell contents, pressure
Turgid
More water enters, water potential of cell solution rises
Water potential out of cell is equal to water potential in
Equilibrium reached
Fully turgid
Cells in lower water potential than cell solution in plants
Water diffuses out of cell through partially permeable membrane
Contents do not push out, flaccid
Plasma membrane eventually pulls away from wall, plasmolysed
External solution fills gap between wall, plasma membrane
Some parts resistant to pulling away from wall
If membrane torn at those points, cells die
Cells in higher water potential than cell solution in human cells
Hypotonic solutions
Water diffuses into the cell
No cell wall, lyses
Cells in lower water potential than cell solution in human cells
Hypertonic solutions
Water diffuses out
Creates