DNA And RNA Flashcards
DNA
Chemical that determines inherited characteristics and contains vast amounts of information in the form of the genetic code
RNA
Help convert genetic code of DNA into functional protein
DNA nucleotide
Phosphate group backbone
Pentose (deoxyribose)
Nitrogenous base
(Adenine, Guanine, Cytosine, Thymine)
RNA nucleotide
Phosphate group backbone
Pentose (ribose)
Nitrogenous base
(Adenine, Guanine, Cytosine, Uracil)
What reaction joins nucleotides?
Condensation
What bond is formed between nucleotides?
Phosphodiester bonds
What bonds keep 2 nucleotide chains together?
Hydrogen bonds
Structure of DNA
Double helix, 2 strands of polynucleotides, run anti parallel to each other, twisted into coil
Bonds in adenine, thymine
2 hydrogen bonds
Bonds in cytosine, guanine molecules
3 hydrogen bonds
What type of molecule is a thymine, cytosine molecule?
Pyramidine molecule (ring)
What type of molecule is adenine and guanine molecule?
Purine molecules (double rings)
Stability of DNA
Stable, phosphodiester bonds are strong covalent bonds
Hydrogen bonds are strong when there are many
Adaptations of DNA
Stable, passes onto next gen without change
2 strands joined with hydrogen bonds, allow them to be easily separated during DNA replication and protein synthesis
Base pairs in helical cylinder, genetic info protected from outside chemical and physical force
Discovery of DNA
Watson and Crick, Cavendish lab in Uni of Cambridge
Completed with Franklin, Pauling,Wilkins
X-rays produced images of DNA
Chargaff’s rule, DNA should have 1:1 ratio of pyramidine, purine bases
Watson led genome project
Comparison between eukaryotic and prokaryotic chromosomes
DNA, v long, linear, associated with histones
DNA, short, circular, not associated with proteins
Histones allow more folding
DNA and chromosomes
Nucleosome, 8 histones with DNA wrapped around twice
Chromosome=DNA molecule and histones
Mitochondrial and chloroplast DNA
Mitochondrial DNA, similar to prokaryotic DNA
Chloroplasts DNA, similar to prokaryotic DNA
Not associated with proteins
Genes
Base seq of DNA, codes for amino acid seq of polypeptide
Locus
Gene occupies fixed position on chromosome
Triplet code
Seq of 3 base code for 1 amino acid
Exons
Code for animo acid seq
Introns
Separate exons by non coding regions
mRNA
Bases complementary to 1 of the DNA strands
Formed in nucleus, moves to ribosomes
Acts as a template upon which proteins are made
Easily broken down, only exists when needed, less wasteful
tRNA
80 nucleotides long Clover leaf structure Shape held together by hydrogen bonds 2 distinctive ends 1 end, amino acid attachment site, other site, anticodon, complementary to mRNA codon Chemically more stable than mRNA Only produced when necessary
Universal
Same codon codes for same amino acid in all organisms
Non overlapping
Each base sequence is only read once
Degenerate
Most amino acids have more than 1 codon