Transgenics Flashcards
1
Q
How many days pseudopregnant should female mouse be for embryo implantation for transgenic mouse making
A
0.5 dpc
2
Q
Blastocyst is how many DPC?
A
3.5
3
Q
2 cell types making up blastocyst
A
Trophoectoderm
Inner cell mass
4
Q
isogenic dna
A
Genomic DNA homologous to endogenous gene in ES cells
5
Q
When should pseudopregnant female be implanted with blastocyst with ES cells?
A
2.5 dpc
6
Q
Site for binding tetracycline
A
tetO
7
Q
Site for binding tamoxifen
A
ER
8
Q
What does CRISPR stand for
A
Clustered Regularly Interspaced Short Palindromic Repeats
