Diagnostic serology - nonrodents

Question 1

How to screen for Treponema cuniculi

Answer 1

Screen with rapid plasma reagin (RPR) card assay that detects reagin (substances released by cells when they are damaged by Treponema infection) Confirm with fluorescent treponemal antibody adsorption (FTA), microhemagglutination (MHA), or particle agglutination assay

Question 2

How does MHA test for Tremponema?

Answer 2

Red blood cells are sensitized with antigens from T. pallidum. The erythrocytes aggregate together to form distinctive patterns on the surface of a microplate wells when exposed to anti-Treponemal serum

Question 3

Primagam measures what for TB testing?

Answer 3

release of gamma interferon produced by stimulated lymphocytes when blood is exposed to TB antigen

Question 4

FeLV SNAP tests what

Answer 4

antigen, not antibody - not affected by FeLV antibodies

Question 5

FIV SNAP test can be influenced by what?

Answer 5

Passive immunity leads to false positives

Question 6

Advantage of C6 antigen SNAP for Lyme

Answer 6

No cross reaction with other tick borne agents. C6 Ab decline after treatment. This test looks for active, ongoing infection

Question 7

heartworm SNAP test detects Ag or Ab?

Answer 7

Ag of adult female

Question 8

What can lead to false negatives on heartworm test

Answer 8

Only male worms present, infection less than 5 months, dogs younger than 7 months