Diagnostic Serology Rodents

Question 1

3 examples of primary tests

Answer 1

a. Immunofluorescence assay (IFA)
b. Immunoenzymatic assays (e.g. ELISA)
c. Multiplex fluorescence immunoassay (MFI)

Question 2

Are primary tests more or less sensitive than secondary or tertiary tests?

Answer 2

yes

Question 3

MFI

Answer 3

Multiplex fluorescent immunoassay

Question 4

Sensitivity

Answer 4

ability of a test to correctly identify POSITIVE samples
Sensitivity (%) = (# true positive results / 
# true positive results + # false negative results) * 100

Question 5

Specificity

Answer 5

ability of a test to correctly identify NEGATIVE samples
(# true-negative results / 
# true-negative results + # false-positive results) * 100

Question 6

Downsides to using high affinity, potent Abs

Answer 6

Unwanted cross reactions

Question 7

Downsides to using weak antibodies

Answer 7

Specific, but not sensitive

Question 8

3 most common pathogens in mice

Answer 8

MNV, MPV, MHV

Question 9

2 most common pathogens in rats

Answer 9

Parvoviruses ( RMV, H-1, RV, RPV), RTV/TMEV