Transcription and translation Flashcards

1
Q

Transformation

A

The change in DNA make up after the cell takes up DNA from it’s environment.

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2
Q

Where are covalent bonds in DNA

A

Between sugars and phosphates

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3
Q

Where are hydrogen bonds found in DNA

A

Between bases, (A&T or C&G)

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4
Q

Watson and Crick

A

Discovered the double helix shape of DNA

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5
Q

Nucleotide

A

Molecule that includes sugar, phosphate group and nitrogenous base. RNA (ribose) DNA ( deoxyribose)

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6
Q

Nitrogenous base

A

What makes each nucleotide different.

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7
Q

DNA bases

A

Adenine, Thymine,Cytosine and Guanine

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8
Q

RNA bases

A

Adenine, Uracil, Cytosine and Guanine

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9
Q

A Gene (molecule biologists)

A

A section of DNA that codes for a protien

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10
Q

When does DNA replication occur?

A

During the S (synthesis) stage of Interphase.

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11
Q

How does DNA replicate

A

It goes from one double helix strand to two strands

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12
Q

3 steps of DNA replication

A
  1. Initiation
  2. Elongation
  3. Termination
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13
Q

Step 1 Initiation

A

Helicase, single strand binding protein, primase, primer, and DNA polymerase

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14
Q

Helicase

A

Unwinds the double helix

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15
Q

Single strand binding protein

A

Holds the two strands of DNA apart so they don’t wind back together.

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16
Q

Primase

A

Attaches to DNA to be copied and makes complementary strand of RNA

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17
Q

Primer

A

Short section of RNA made to start the replication process

18
Q

Polymerase

A

can only lengthen existing strand of primer to form new DNA.

19
Q

Step 2 Elongation

A

DNA polymerase, leading strand and lagging strand.

20
Q

DNA polymerase

A

Lengthens the DNA strand in the 5’ to 3’ direction.

21
Q

Leading strand

A

Seamlessly follows helicase down the DNA in the 5’ - 3’ direction

22
Q

Lagging strand

A

DNA is replicated in short strans in the opposite direction of the leading strand. Also in the 5’-3’ direction

23
Q

Okazaki fragments

A

Shorts stans of DNA on the lagging section between the RNA primers

24
Q

Step 3. Termination

A

DNA polymerase replaces short RNA primers with DNA

Ligase connects Okazaki segments

25
Proof reading
DNA polymerase proof reads each DNA as it copies. There are about 1:1 billion mistakes made
26
Telomere
Postpone the erosion of genes at the end of a DNA strands due to shortening.
27
Telomerase
Is the enzyme that catalyzes replacement of telomere in germ cells
28
Semi-conservative process
One parent strand of DNA is conserved and one strands is a new daughter strand.
29
DNA
Carriesa hereditary info 2 strands Deoxyribose A,T,C,G
30
RNA
Builds proteins 1 strand Ribose A,U, C,G
31
3 steps to make a protein
Transcription RNA Processing Translation
32
Transcription
Making a copy of the gene of DNA
33
RNA processing
Taking pre-mRNA and building a molecule that can build a protein, mRNA
34
Translation
Takes place in the ribosome, taking info from mRNA to make a protein
35
3 kinds of RNA
mRNA tRNA rRNA
36
mRNA
Messenger, made during transcription and RNA processing. Carries the code out of the nucleus to the cytoplasm where the protein is built.
37
tRNA
Transfer: helps build protein shaped like a 4 leaf clover anti-codon that attacked to mRNA
38
rRNA
Ribosomal: 2 parts, small and large sub units. The two parts come together to house the mRNA and tRNA so they can make the protein
39
3 binding sites on tRNA
P:carries polypeptite A: holds amino acid chain E: exit site
40
Introns
Non-codong regions of the RNA which need to be cut out before a protien is built.
41
RNA splicing
Cutting out endtrons
42
Exton
The coding region of DNA