DNA Replication Flashcards
DNA
Contains genetic material
Transformation
A change in genotype and phenotype due to assimilation of foreign DNA
DNA pairing
Adenine and guanine = purines
Thymine and cytosine = pyrimidines
Semi-conservative model
When DNA replicates there is one strand conserved from the parent DNA and the new daughter strands forms next to it.
Helicase
An enzyme that unwinds the double helix at the origin of replication
Replication bubble
The point where the DNA is split, each end are a fork in the DNA strands
Single strand binding protein
Holds the DNA strands apart so they don’t coil back up
Primase
Binds to the DNA and makes a complementary strand of RNA called primer.
DNA polymerase
Lengthens the primer RNA and builds new DNA based on the DNA template from the parent cell.
Topoisomerase
Keeps the DNA from coiling back on the double helix.
Elongation
DNA polymerase can only lengthen in one direction from 5’ to 3’ direction
2 strands
leading
lagging .
leading
Starts at helcase stran and is seamless
lagging
DNA polymerase works away fron replication fork (5’-3’ direction), makes short, okazaki fragments joined by DNA ligase.
Termination
Diferent DNA polymerases replaces RNA bases with DNA bases, then ligase connects okazaki fregmants to complete the strand.