Transcription Flashcards

1
Q

Where does transcription occur?

A

Nucleus

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2
Q

Write the RNA codon for this DNA code:
3’ ATCGTAACC 5’

A

Codon: 5’ UAGCAUUGG 3’

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3
Q

Name the 3 phases of Transcription

A

Initiation, elongation, termination

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4
Q

What does the initiation phase of transcription involve?

A

RNA polymerase binds promotor region

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5
Q

What transcribes rRNA?

A

RNA polymerase I

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6
Q

What transcribes mRNA?

A

RNA polymerase II

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7
Q

What transcribes tRNA?

A

RNA polymerase III

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8
Q

What does the elongation phase of transcription involve?

A

Complementary base pairs added 5’ to 3’, DNA read 3’ to 5’

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9
Q

What does the termination step of transcription involve?

A

Stop code reached —> hairpin loop created

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10
Q

What RNA molecules are involved in transcription?

A

.rRNA, mRNA, tRNA

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11
Q

What does rRNA consist of? What is its function and where does it occur?

A

Small (40S) + large (60S) subunits = 80S ribosome in nucleolus

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12
Q

What does mRNA contain?

A

Codon (3 bases; ea. codon codes an AA)

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13
Q

What does tRNA consist of? What is most important?

A

4 loops; anticodon and acceptor stem

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14
Q

What does tRNA’s anticodon loop do?

A

Base pairs w/ mRNA

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15
Q

What does tRNA’s acceptor loop do?

A

Binds AA (coded for by anticodon)

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16
Q

What enzyme binds AA to tRNA? (What does it require)

A

Aminoacyl-tRNA synthetase, (ATP)

17
Q

Write the mRNA codon for this RNA codon:
5’ UAGCAUUGG 3’

A

5’ UAGCAUUGG 3’

18
Q

Write the tRNA anticodon for this mRNA codon:
5’ UAGCAUUGG 3’

A

Anticodon: 3’ AUCGUAACC 5’

19
Q

What 3 steps occurs in Message Processing? What are steps 2 + 3 done by?

A
  1. Guanylated phosphate cap + polyA tail (200 adenine residues) are added
  2. Introns cut out by splicosomes
  3. Exons spliced together by ribozymes