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Biochem 1 Final > Enzyme Facts > Flashcards

Enzyme Facts Flashcards

1
Q

What is the function of enzymes?

A

Lower activation energy of rxns

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2
Q

Enzymes biochemically are _____, except for _____.

A

Proteins, ribozymes

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3
Q

Enzymes catalyze _____ rxn / rxn type, exhibiting what?

A

Specific, optimal specificity

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4
Q

Enzymes only act on what isomer of an AA? Of a sugar?

A

L-isomer, D-isomer

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5
Q

Enzymes _____ used up in the rxn

A

Are NOT (E + S —> ES —> E + P)

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6
Q

True/False: Enzymes alter the equilibrium constants

A

False

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7
Q

True/False. Enzymes change the ∆G of the rxn

A

False

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8
Q

∆G

A

Free energy

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9
Q

Exergonic rxns have a _____ and occur _____.

A

-∆G, spontaneously (no energy used/needed)

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10
Q

Endergonic rxns have a _____ and require _____.

A

+∆G, energy input

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11
Q

What 2 factors affect enzymes?

A

Temp + pH

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12
Q

Why does temp affect enzymes?

A

Enzymes work over a very narrow range of temp in humans

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13
Q

What happens to rxns when ^ temp? What happens to enzymes at temp > 102˚F?

A

^ rxn rate. Proteins (enzymes) denature

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14
Q

What happens to rxn rates when temp < 98˚F?

A

Slows down

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15
Q

Why does pH affect enzymes?

A

Enzymes have an optimal pH

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16
Q

Pepsin is found in _____ and is an example of an enzyme with what optimal pH?

A

Stomach, Acidic: 1.5 - 2.0

17
Q

Carbonic Anhydrase is found in _____ and is an example of an enzyme with what optimal pH?

A

RBCs, Neutral: 7.3 - 7.4

18
Q

Trypsin is found in _____ and is an example of an enzyme with what optimal pH?

A

Sm Intestine, Basic: 8.0 - 9.0

19
Q

Draw + label the Michaelis-Menten Curve. What are the typical units for the X + Y axis?

A
20
Q

How is Km determined?

A

Km: [S] when rate = 1/2 Vmax

21
Q

What is Km?

A

Estimated physiological [substrate]

22
Q

What is Vmax?

A

Max rxn velocity

23
Q

Describe the effects of [S] on activity of enzymes. Does this happen to all enzymes?

A

Michaelis-Menten Curve
(E+S —> ES —> E+P)
MANY, but not all, enzymes

24
Q

What plot is Lineweaver-Burke plot? Draw + label the plot.

A

Double reciprocal

25
Q

Name 2 experimental plots to determine Km + Vmax. Which plot is better?

A

Lineweaver-Burke Plot, Eadie-Hofstee

26
Q

What plot is Eadie-Hofstee? Draw + label the plot

A

Single reciprocal

27
Q

What is the disadvantage of Lineweaver-Burke?

A

Sm experimental error can = lrg error in graphically determined values

28
Q

What determines Km in Eadie-Hofstee?

A

Slope

Biochem 1 Final (22 decks)

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