Transcription Flashcards
Sequence of 3 bases in an mRNA are called
Codons
Start codon
AUG
Stop codons
UAA
UAG
UGA
tRNA has 3 nucleotide bases called what
anticodon
The process of copying the sequence of one strand of DNA (Template strand)
DNA Transcription
mRNA copies what strand
Template strand
Key element in promoter
TATA box
3 kinds of polymerase
Polymerase I (A)
Polymerase II (B)
Polymerase III (C)
Polymerase that catalyzes the formation of rRNA
Polymerase I
Polymerase that catalyzes the formation of mRNA
Polymerase II
Polymerase that catalyzes the formation of tRNA
Polymerase III
Regions in DNA where RNA polymerases must bind to begin the transcription of RNA
Promoters
Element of Promoter and a.k.a Hogness Box
TATA box
Can initiate transcription with the use of INR and DPE
TATAless promoter
INR means
Initiator Element
DPE means
Downstream Promoter Element
Sequence of INR
TCA (G or T) T( T or C)
Sequence of DPE
(A or G) G (A or T) CGTG
Forms an apparatus that binds DNA and initiates transcription
Transcription Factors
Transcription Steps
Initiation
Elongation
Termination
Will recognize the TATA box and bind to it
Transcription factor IID
Binds to TATA box and recruits other transcription factors
TFIID
Stabilizes the IID so it wont be removed
TFIIA
Aids in starts sign selection and link the RNA Polymerase II
TFIIB
Destabilizes non-specific interactions of RNA Polymerase II and DNA, so it wont get attached to the other parts of the DNA
TFIIF
Will melt the promoter, since it is a helicase and will activate RNA Polymerase II since it is a kinase
TFIIH
Enhances promoter melting and semi-controls the function of TFIIH
TFIIE
When does Initiation Step end
At Elongation Complex
What is added in the Elongation step
Nucleotides
Termination Signal which is found after the stop codon
AAUAAA
Cleaves the RNA from RNA Polymerase II
CPSF
CPSF meaning
Cleavage and Polyadenylation Specificity Factor
The RNA that is the direct copy of the DNA
Primary Transcript
Happens at the 5’ end of the RNA and GTP is usually added shortly after RNA Polymerase makes the 5’ end primary transcript
RNA Capping
Modifies the 3’ end of the primary transcript by the addition of a string of A’s
Polyadenylation
Forms spliceosome
snRNPS
What is added to the 5’ end of a newly copied mRNA
Guanosine Triphosphate cap
What is added to the 3’ end of a newly copied mRNA
Poly A tail