DNA Replication Flashcards

1
Q

Double stranded molecule with a double helix structure

A

DNA Structure

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2
Q

3 components of DNA structure

A

Phosphate group
Sugar
Nitrogenous Base

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3
Q

Process by which double-stranded DNA molecule is copied to produce two identical DNA molecules

A

DNA Replication

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4
Q

Replication units called ________, which forms bubbles and are stretches of DNA with a single origin of replication

A

Replicons

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5
Q

3 models of DNA Replication

A

Semiconservative
Conservative
Dispersive

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6
Q

DNA replication model where each of the two molecule created would be a complex of a parent and daughter strand

A

Semiconservative

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7
Q

DNA replication model where the process would create a brand-new DNA double helix made of two daughter strands while the parental chain would stay together

A

Consevative

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8
Q

DNA replication model where the process would create two DNA double-chains, each of them with parts of both parents and daughter molecules

A

Dispersive

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9
Q

Which DNA replication model is the correct model

A

Semiconservative

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10
Q

Who proved that the Semiconservative model of DNA Replication is the correct model

A

Matthew Meselson
Franklin Stahl

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11
Q

Synthesizes the Leading strand and replicates towards the replication fork

A

Continuous Replication

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12
Q

Synthesizes the Lagging strand
Occurs in short segments called Okazaki fragments
Replicates away from the replication fork

A

Discontinuous Replication

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13
Q

5 Enzymes in DNA Replication

A

Helicase
Primase
Clamp Protein
DNA Polymerase
Ligase

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14
Q

Which enzyme unwinds the DNA double helix

A

Helicase

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15
Q

Which enzyme synthesizes short oligonucleotides (primers)

A

Primase

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16
Q

Which enzyme allows the leading strand to be threaded through

A

Clamp Protein

17
Q

Which enzyme joins the assembled nucleotides and acts as a builder because it adds new nucleotides

A

DNA Polymerase

18
Q

Joins Okazaki fragments in the lagging strands; Glues DNA fragments together

A

Ligase

19
Q

Synthesized continuously as a single strand from the point of origin toward the opening replication fork

A

Leading Strand

20
Q

Synthesized discontinuously against overall direction of replication

A

Lagging Strand

21
Q

Series of short segments on the lagging strand

A

Okazaki Fragments

22
Q

Identify which Step # in DNA Replication:

Helicase unwinds the DNA and separates the strands

A

Step 1

23
Q

Identify which Step # in DNA Replication:

Single-stranded binding proteins attach and keep the 2 DNA strands separated and untwisted

A

Step 2

24
Q

Identify which Step # in DNA Replication:

Primase creates the primers on both strands

A

Step 3

25
Q

Identify which Step # in DNA Replication:

DNA polymerase can then add the new nucleotides

A

Step 4

26
Q

Identify which Step # in DNA Replication:

DNA polymerase proofreads, checks activity, and replaces incorrect bases

A

Step 5

27
Q

Identify which Step # in DNA Replication:

Replication continues. Synthesis of Continuous and Discontinuous strands

A

Step 6

28
Q

Identify which Step # in DNA Replication:

Enzymes remove RNA primers

A

Step 7

29
Q

Where does energy for bonding come from

A

Nucleotides (contains ATP)

30
Q

RNA primer is built by?

A

Primase

31
Q

Serves as starter sequence for DNA Polymerase III

A

RNA primer