HistoPath Laboratory Flashcards
Used to label non-frosted slides
Diamond Pen
Disposable type of molder
Paper boat
Describes the characteristics or appearance of tissue macroscopically
Gross examination
Automated Machines cannot perform what fixation step?
Decalcification
Paraffin Dispenser temperature
2-4 degrees higher than the melting point of paraffin
Freezer thermal requirement
-20 degrees Celcius
Refrigerator thermal requirement
4 degrees Celcius
Main parts of Microtome
Block Holder
Knife Holder
Pawl and Feedwheel Mechanism
Microtome that is most commonly used and is designed for tissue sections that are embedded using paraffin wax
Rotary Microtome
Microtome used for STAT diagnosis where a rotary microtome is kept in a cold chamber
Cryostat
Cryostat temperature
-5 to -30 degrees Celcius
Oldest and simplest type of microtome that is invented by Trefall
Rocking Microtome
Microtome used for cutting large tissue sections with Cellulose Nitrate as the embedding medium
Sliding Microtome
Embedding Medium used for Ultrathin Microtome
Epoxy Resin
Measurement of cut section from an Ultrathin Microtome
60 to 100 nanometers
Flotation bath temperature
5 to 10 degrees lower than paraffin wax melting point
Slide dryer temperature
5 to 10 degrees higher than paraffin wax melting point
How long should slides be placed on the slide dryer
3 to 5 mins only
Microscope commonly used during the Histology Laboratory
Brightfield
Sample requirement for Darkfield Microscope
Unstained and freshly prepared
Sample requirement for Phase Contrast Microscope
Colorless or Transparent
