HistoPath Laboratory Flashcards

1
Q

Used to label non-frosted slides

A

Diamond Pen

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2
Q

Disposable type of molder

A

Paper boat

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3
Q

Describes the characteristics or appearance of tissue macroscopically

A

Gross examination

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4
Q

Automated Machines cannot perform what fixation step?

A

Decalcification

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5
Q

Paraffin Dispenser temperature

A

2-4 degrees higher than the melting point of paraffin

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6
Q

Freezer thermal requirement

A

-20 degrees Celcius

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7
Q

Refrigerator thermal requirement

A

4 degrees Celcius

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8
Q

Main parts of Microtome

A

Block Holder
Knife Holder
Pawl and Feedwheel Mechanism

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9
Q

Microtome that is most commonly used and is designed for tissue sections that are embedded using paraffin wax

A

Rotary Microtome

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10
Q

Microtome used for STAT diagnosis where a rotary microtome is kept in a cold chamber

A

Cryostat

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11
Q

Cryostat temperature

A

-5 to -30 degrees Celcius

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12
Q

Oldest and simplest type of microtome that is invented by Trefall

A

Rocking Microtome

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13
Q

Microtome used for cutting large tissue sections with Cellulose Nitrate as the embedding medium

A

Sliding Microtome

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14
Q

Embedding Medium used for Ultrathin Microtome

A

Epoxy Resin

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15
Q

Measurement of cut section from an Ultrathin Microtome

A

60 to 100 nanometers

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16
Q

Flotation bath temperature

A

5 to 10 degrees lower than paraffin wax melting point

17
Q

Slide dryer temperature

A

5 to 10 degrees higher than paraffin wax melting point

18
Q

How long should slides be placed on the slide dryer

A

3 to 5 mins only

19
Q

Microscope commonly used during the Histology Laboratory

A

Brightfield

20
Q

Sample requirement for Darkfield Microscope

A

Unstained and freshly prepared

21
Q

Sample requirement for Phase Contrast Microscope

A

Colorless or Transparent