Tissue Processing Flashcards
1
Q
What are the 3 main characteristics of tissue samples used for observation with a microscope?
A
- well-preserved during fixation using formalin
- sufficiently thin using a microtome (1-7 μm)
- contrasted using staining
2
Q
What are the 5 major steps of tissue processing?
A
- fixation (formalin)
- dehydration (ascending % of alcohol)
- clearing (xylene)
- infiltration (fluid)
- embedding (solid paraffin wax)
3
Q
What is the microtome used for?
A
sectioning paraffin-embedded tissue into 1-7 μm (usually 5) thick segments
4
Q
What process is done to the tissue after it goes through the microtome?
A
staining
