Pathology of the Hematopoietic System Flashcards
What are the 3 main sources of lymphocytes?
yolk sac —> fetal liver —> bone marrow
What is the difference between primary and secondary lymphoid organs?
PRIMARY: sites of lymphocyte development
- thymus
- bursa
- Peyer’s patches
- bone marrow
SECONDARY: sites where lymphocytes respond to trapped antigens to launch immune response
- tonsils
- spleen
- lymph nodes
- Peyer’s patches
- bone marrow
How does the composition of bone marrow change with age?
YOUNG: active, red marrow
OLD: nonhematopoietic tissue, mainly fat; yellow
Where does hematopoiesis usually occur? In adults specifically?
throughout flat and long bones
pelvis, sternum, ribs, vertebrae, and proximal humerus and femur
What are the major 2 events that stem cells undergo during differentiation?
- hematopoiesis
- lymphopoiesis
What is the main regulator of erythropoiesis? When and where is it produced? What are 3 additional stimlators?
erythropoietin (Epo) regulated the production of RBCs
during hypoxia in the kidney and liver
- ILs
- CSFs (colony stimulating factors)
- hormones
What are myeloid cells? What is the production of these cells called? Why are these cells produced?
granulocytic (granulopoiesis) and monocytic (monocytopoiesis) cells
myelopoiesis - cells that migrate from blood to tissue to induce inflammation in response to microorganisms (host defense)
What are the functions of the cells produced during myelopoiesis (granulopoiesis + monocytopoiesis)?
NEUTROPHILS and MONOCYTE-DERIVED MACROPHAGES: phagocytosis and microbicidal activity (bacteria)
EOSINOPHILS and BASOPHILS: parasiticidal activity, allergic reactions
MACROPHAGES: antigen processing and presentation, cytokine production
What are the 3 stimulators of granulopoiesis and monocytopoiesis?
- ILs
- G-CSF (granulocyte colony-stimulating factor)
- GM-CSF (granulocyte-macrophage colony-stimulating factor)
What regulates megakaryopoiesis (thrombopoiesis)? Where is it produced?
thrombopoietin (Tpo)
liver
(megakaryocyte —> platelet)
What are 4 other cells found in the bone marrow?
- lymphocytes (1-10%)
- plasma cells (<1%)
- stromal cells: reticular cells, adventitial cells, adipocytes
- osteoblasts/osteoclasts
What 2 diagnostics are used to examine bone marrow? In what 4 situations is this done?
bone marrow aspirates and core biopsies
- unexplained cytopenias (any non-regenerative anemia0
- maturation defects or morphological abnormalities in blood cells
- potential myeloproliferative/lymphoproliferative disease
- potential malignancies metastatic to bone marrow
What needs to also be submitted with bone marrow aspirated and core biopsies?
CBC
Bone marrow is located in multiple sites, but responds as a single tissue. Where are aspirates and core biopsies typically taken from dogs and cats, horses, and cattle?
(from any bone with red marrow)
DOGS/CATS: proximal femur, iliac crest, proximal humerus
HORSES: sternum
CATTLE: proximal rib
Bone marrow aspirates and core biopsy:
Who interprets bone marrow aspirates/smears? What 3 things are they important for observing?
clinical pathologist
- cellular morphology and maturation
- myeloid to erythroid ratio (M:E)
- primary or metastatic neoplasia
Who interprets bone marrow core biopsies? What 5 things are they important for observing?
anatomical pathologist
- ratio of fat to hematopoietic cells
- myeloid to erythroid ratio (M:E)
- adequacy of iron
- stomal elements (myelofibrosis, scaring)
- primary or metastatic neoplasia
Bone marrow aspirate on cytology:
What is the normal myeloid-to-erythroid ratio?
3:1
(bone marrow core biopsy)
What are the 5 predominant bone marrow patterns?
- hyperplasia
- hypoplasia
- myelitis/necrosis
- dysplasia/neoplasia
- myelofibrosis
How do the 3 types of hyperplastic pattern change depending on the stimulus?
ERYTHROID hyperplasia - response to anemia
MEGAKARYOCYTIC hyperplasia - response to low platelets
MYELOID hyperplasia
- neutrophilia - bacterial infections, tissue necrosis
- eosinophilia - parasites, hypersensitivities
- monocytosis - chronic infections, specific agents
What is the pathogenesis behind the bone marrow hyperplasia pattern?
decrease in cell numbers in blood caused by increased peripheral demand or the adequate number of hypofunctional cells in peripheral blood leads to increased cell production in the marrow in response to poietins and interleukins
What is the gross finding in bone marrow hyperplasia?
red marrow replacing yellow marrow (fat) at metaphysis and endosteal surface of the diaphysis