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Laws, Regulations, and Policies > The Guide Ch3 > Flashcards

The Guide Ch3 Flashcards

1
Q

How many fresh air changes per hour in an animal housing room is an acceptable guideline to maintain macroenvironmental air quality?

A

10-15 AC/H

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2
Q

Exposure to sound louder than 85 dB can have which effects?

A

Eosinopenia in rodents

Increased blood pressure in nonhuman primates

Reduced fertility in some animals

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3
Q

True or False: All poikilothermic animals are aquatic.

A

False - reptiles

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4
Q

Which type of waste should be removed regularly?

a. conventional
b. biologic
c. hazardous
d. all of the above

A

d. all of the above

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5
Q

True or False: The Guide can be used to determine the housing requirements of all species used in research.

A

False - there are many species used in research that are not specifically addressed in the Guide

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6
Q

What do ventilated caging systems provide that static caging systems don’t?

A

direct continuous exchange of air

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7
Q

Define the microenvironment

A

The immediate physical environment surrounding the animal

i.e….the environment in the primary enclosure such as the cage, pen, or stall

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8
Q

Define the macroenvironment

A

The physical environment of the secondary enclosure

i.e…..a room, a barn, or an outdoor habitat

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9
Q

Define the TNZ

A

Thermoneutral Zone: the ambient temperature range in which thermoregulation occurs without the need to increase metabolic heat production or activate evaporative heat loss mechanisms

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10
Q

What are the areas called above and below the TNZ and what happens outside the TNZ?

A

The lower and upper critical temperatures (LCTs & UCTs)

To maintain body temperature under a given environmental temperature animals adjust physiologically (incl metabolism) and behaviorally (nesting)

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11
Q

What is the TNZ for the following animals?

Mice

Rats

Gerbils

Rabbits

Cats & Dogs

A

Mice = 26-34C

Rats = 26-30C

Gerbils = 28-32C

Rabbits = 15-20C

Cats & Dogs = 20-25C

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12
Q

T/F: In general, dry-bulb temperatures should be set above at the animals’ LCT to avoid heat stress.

A

False: to avoid heat stress temperatures should be set BELOW the LCT, therefore animals should be provided with adequate resources for thermoregulation to avoid cold stress

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13
Q

Which animals generally require a temperature gradient in their environment?

A

Poikilotherms and young birds of some species require a thermal gradient in their primary enclosure to meet basic physiologic processes

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14
Q

What are the recommended dry-bulb temperatures for the following animals?

Mouse, rat, hamster, gerbil, GP

Rabbit

Cat, dog, NHP

Farm animals, poultry

A

Mouse, rat, hamster, gerbil, GP = 20-26C (68-79F)

Rabbit = 16-22C (61-72F)

Cat, dog, NHP = 18-29C (64-84F)

Farm animals, poultry = 16-27C (61-81F)

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15
Q

What is the acceptable range of relative humidity for most mammalian species?

A

30-70%

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16
Q

If exhaust air is to be recycled, how should it be treated?

A

The exhaust air to be recycled should be filtered, at a minimum, with 85-95% ASHRAE efficient filters to remove airborne particles before it is recycled.

American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE): Assign Minimum Efficiency Reporting Values (MERV) rating to rate the effectiveness of air filters

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17
Q

What may happen to chickens if they are sustained in low light or dark conditions?

A

The will not eat

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18
Q

What light level parameters appear to be sufficient for animal care and do not cause clinical signs of phototoxic retinopathy in albino rats?

A

325 lux (30-ft candles) approximately 1 meter (3.3 ft) above the floor

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19
Q

Light intensity in what range above the light intensity under which an animal was raised has been reported to be near the threshold of retinal damage in some albino rats?

A

130-270 lux

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20
Q

For animals that have been shown to be susceptible to phototoxic retinopathy, light should be at what intensity in the room at cage level?

A

130-325 lux

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21
Q

How does light intensity decrease with distance from the source?

A

Light intensity decreases with the square of the distance from its source

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22
Q

When given the choice, rodents prefer what kind of flooring?

A

When given the choice, rodents prefer SOLID floors (with bedding) to grid or wire-mesh floors

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23
Q

At what frequency should enrichment programs be reviewed by the IACUC, researchers, and veterinarian?

A

On a “regular basis”

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24
Q

T/F: Enrichment affects animal phenotype and may affect the experimental outcome.

A

True: enrichment should be considered an independent variable and appropriately controlled

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25
Q

T/F: Animals maintained in outdoor runs, pens, or other large enclosures should have protection from extremes in temperature or other harsh weather conditions and adequate opportunity for retreat.

A

False: Animals MUST be provided these protections

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26
Q

At a minimum, animals must have enough space to do what?

A

Express their natural postures and postural adjustments without touching the enclosure walls or ceiling, be able to turn around, and have ready access to food and water.

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27
Q

T/F: Floor space taken up by food bowls, water containers, litter boxes, and enrichment devices should not be considered part of the floor space.

A

True

The Guide differs from AWRs, which allows cat litter boxes to be included in floor space calculation, provided it is “properly cleaned and sanitized.”

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28
Q

T/F: Adjustments to the amount and arrangement of space recommended by the Guide should be reviewed and approved by the IACUC, and should be based on performance indices related to animal well-being and research quality.

A

True

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29
Q

According to the Guide, at what frequency should dogs and cats housed individually or in smaller enclosures be allowed to exercise and be provided with positive human interaction?

A

No specific frequency is provided

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30
Q

T/F: Determination of the appropriate cage size is based solely on the body weight of the animal

A

False: Determination of the appropriate cage size is NOT based on the body weight of the animal alone…professional judgment is paramount in making such determinations

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31
Q

At what frequency should the need for single housing be reviewed by the IACUC and veterinarian?

A

On a “regular basis”

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32
Q

At what frequency should animals be fed palatable, uncontaminated diets that meet their nutritional and behavioral needs?

A

At least daily, or according to their particular requirements, unless the protocol requires otherwise

33
Q

What is a natural-ingredient diet?

What are the storage conditions?

A

Natural-ingredient diets are formulated with agricultural products and byproducts and are commercially available for all common lab animal species. The nutrient composition of ingredients varies and the diets may contain low levels of naturally occurring or artificial contaminants

Storage of natural-ingredient diets at less than 21C (70F) and below 50% relative humidity is recommended.

34
Q

What is the shelf-life of most natural-ingredient diets if they are properly stored?

A

6 months after milling

Nonstabilized vitamin C in manufactured feeds generally has a shelf life of only 3 months

35
Q

What is a certified diet?

A

Certified diets are assayed for contaminants and are generally used in preclinical toxicology studies conducted in compliance with FDA GLP standards

36
Q

What are purified diets?

What are the proper storage conditions?

A

Purified diets are refined such that each ingredient contains a single nutrient or nutrient class. They have less nutrient concentration variability and the potential for chemical contamination is lower.

Storage at 4C is generally recommended

37
Q

What are chemically defined diets?

How should they be stored?

A

Chemically defined diets contain the most elemental ingredients available, such as individual amino acid or specific sugars

They should be stored at 4C

38
Q

How should open bags of food be stored?

A

Opened bags of food should be stored in vermin-proof containers to minimize contamination and to avoid the potential spread of pathogens

39
Q

What is the problem with using cedar shavings for bedding?

A

The are not recommended because they emit aromatic hydrocarbons that induce hepatic microsomal enzymes and cytotoxicity and have been reported to increase the incidence of cancer

40
Q

What distance should bags of bedding and food be stored from walls?

A

A “sufficient distance” to facilitate cleaning

41
Q

Define sanitation

A

The maintenance of environmental condtions conducive to heatlh and well-being

Involves bedding change, cleaning and disinfection

42
Q

Define cleaning

A

Removing excessive amounts of excrement, dirt, and debris

43
Q

Define disinfection

A

Reduces or eliminates unacceptable concentrations of microorganisms

44
Q

T/F: There is no absolute minimal frequency of bedding changes

A

True: this is determined by professional judgement and consultation between the investigator and animal care personnel

Soild bedding should be removed and replaced as often as necessary to keep the animals clean and dry and to keep pollutants at low enough concentrations to prevent irritation to mucous membranes

45
Q

In general, enclosures and accessories should be sanitized at what frequency?

Solid-bottom caging, bottles and sipper tubes usually require sanitation at what frequency?

A

Every 2 weeks for enclosures & accessories

Once weekly for solid-bottom caging, bottles and sipper tubes

The Guide notes that some types of cages and housing systems may require less frequent cleaning or disinfection

46
Q

Effective disinfection can be achieved with wash and rine water at what temperature?

From what location in the system is that temperature taken?

A

143-180F

The traditional 180F temperature refers to water in the tank or in the sprayer manifold

47
Q

T/F: Emergency veterinary care must be available for work hours, on weekends, and on holidays.

A

True

48
Q

What information should be included on identification cards placed on rooms, racks, pens, or stalls?

A

Source of the animal

Strain or stock

Names and contact information for responsible investigators

Pertinent dates (birth, arrival, etc.)

Protocol number

49
Q

T/F: As a method of ID of small rodents, toe-clipping should be used only when no other individual ID method is feasible and it may be the preferred method for neonatal mice up to 7 days of age.

A

True: it appears to have few adverse effects on behavior and well-being of animals this age, especially if it can be combined with genotyping

50
Q

Define life support system with regard to aquatic animals

A

The physical structure used to contain the water and the animals as well as the ancillary equipment used to move and/or treat the water

51
Q

What are the three general categories of life support systems?

A
  1. Recirculating - water is moved around a system
  2. Flow-through - water is constantly replaced
  3. Static - water is stationary and periodically replenished or replaced
52
Q

What are the four general categories of water available for housing aquatic organisms?

A
  1. Treated wastewater: municipal tap water
  2. Surface water: rivers, lakes, oceans
  3. Protected water: well or aquifer water
  4. Artificial water: RO or distilled water
53
Q

What is the function of the biologic filter (biofilter) in a life-support system?

A

Promote the conversion of ammonia to nitrite and nitrate via nitrifying bacteria

Nitrosomas spp convert ammonia to nitrite

Nitrobacter spp convert nitrite to nitrate

54
Q

T/F: Generally, schooling fish species are housed with conspecifics, and many amphibians, especially anuran species, may be group housed

A

True

55
Q

What is the typical stocking density of Danio rerio?

A

5 adult fish per liter of water

56
Q

What is the typical stocking density of Xenopus laevis?

A

2 liters of water per frog

57
Q

T/F: Latex gloves have been associated with toxicity in some amphibians

A

True

58
Q

How is disinfection typically accomplished in aquaculture systems?

A

Water treatment (filtration and application of UV light or ozone)

Water changes

59
Q

Excessive algal growth may be an indication in elevated levels of what elements?

A

Nitrogen and phosphorus, especially in the presence of light

60
Q

What 3 factors determine the frequency of cleaning and disinfection of aquatic life support systems?

A

Water quality

Adequate viewing of animals

Animal health monitoring

61
Q

What are some ID methods used for aquatic organisms?

A

Fin clipping

Genetic testing

ID tags

SC injections of elastomeric or other materials

Individual transponder tags

External features such as individual color patterns

62
Q

What is the recommended dry bulb temperature for mice?

For what other species is this range recommended?

A

20-26 C

Rat, hamster, gerbil, guinea pig

63
Q

What is the recommended dry bulb temperature for rabbit?

A

16-22 C

64
Q

What is the recommended dry bulb temperature for dog?

For what other species is this range recommended?

A

18-29 C

Cat, NHP

65
Q

What is the recommended dry bulb temperature for farm animals?

For what other animals is this range recommended?

A

16-27 C

66
Q

What is the recommended floor area in square inches for mice housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches?

A

<10 g: 6

up to 15 g: 8

up to 25 g: 12

> 25 g: 15

Female with litter: 51

minimum height for mice: 5 inches

67
Q

What is the recommended floor area in square inches for rats housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches?

A

< 100 g: 17

up to 200 g: 23

up to 300 g: 29

up to 400 g: 40

up to 500 g: 60

> 500 g: 70

Female with litter: 124

Minimum cage height: 7

68
Q

What is the recommended floor area in square inches for hamsters housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches?

A

< 60 g : 10

up to 80 g: 13

up to 100 g: 16

> 100 g: 19

Minimum cage height 6

SAME REQUIREMENTS AS AWA

69
Q

What is the recommended floor area for guinea pigs in square inches housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches?

A

up to 350 g: 60

> 350 g: 101

Minimum cage height 7

SAME AS AWA REQUIREMENTS

70
Q

What is the recommended floor area in square feet for rabbits housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches?

A

< 2 kg: 1.5

2-4 kg: 3

4 -5.4 kg: 4

> 5.4 kg: 5

Same as AWA requirements

Minimum height: 16 inches (2 inches higher than AWA)

71
Q

What is the recommended floor area in square feet for cats housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches?

A

up to 4 kg: 3

> 4 kg: 4

Minimum height 24 inches

Same as AWA requirements

72
Q

What is the recommended floor area in square feet for dogs housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches?

A

< 15 kg: 8

up to 30 kg: 12

> 30 kg: 24

Or AWA requirement, whichever is greater

The Guide prefers enclosures that allow greater freedom of movement and unrestricted height

73
Q

What is the recommended floor area in square feet for pigeons, quail, and chickets (in order of increasing weight range)? What is the recommended minimum cage height in inches?

A

PIgeons: 0.8 ft2

Quail: 0.25 ft2

Chickens < 0.25 kg: 0.25 ft2

up to 0.5 lkg: 0.5

up to 1.5 kg: 1.0

up to 3.0 kg: 2.0

>3.0 kg: 3.0

Cage height should be sufficient for animals to comfortably stand erect with their feet on the floor.

74
Q

What is the recommended floor area in square feet for NHPs (excluding chimpanzees) housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches for each weight group?

A

Group 1 - up to 1.5 kg: 2.1 ft2 x 30 in

Group 2 - up to 3 kg: 3.0 ft2 x 30 in

Group 3 - up to 10 kg: 4.3 ft2 x 30 in

Group 4 - up to 15 kg: 6.0 ft2 x 32 in

Group 5 - up to 20 kg: 8 ft2 x 36 in

Group 6 - up to 25 kg: 10 ft2 x 46 in

Group 7 - up to 30 kg: 15 ft2 x 46 in

Group 8 - > 30 kg: 25 ft2 x 60 in

75
Q

What is the recommended floor area in square feet for chimpanzees housed in groups, in order of increasing weight range? What is the recommended minimum cage height in inches for each weight group?

A

up to 10 kg: 15 ft2 x 60 in

> 10 kg: 25 ft2 x 84 in

76
Q

What is the recommended floor area in square feet for swine housed individually, in order of increasing weight range?

A

< 15 kg: 8

up to 25 kg: 12

up to 50 kg: 15

up to 100 kg: 24

up to 200 kg: 48

> 200 kg: 60

77
Q

What is the recommended floor area in square feet perswine for a group of 2-5, in order of increasing weight range?

A

< 25 kg: 6

up to 50 kg: 10

up to 100 kg: 20

up to 200 kg: 40

> 200 kg: 52

78
Q

What is the recommended floor area in square feet per swine for a group greater than 5, in order of increasing weight range?

A

< 25 kg: 6

up to 50 kg: 9

up to 100 kg: 18

up to 200 kg: 36

> 200 kg: 48

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