Test 2, Deck 9

Question 1

Select the environment in which E.coli would produce B-galactosidase
A with lactose without glucose
B without lactose and glucose
C with lactose and glucose

Answer 1

A with lactose without glucose
Requires lactose to remove lac operon, transcription requires glucose to be low to increase cAMP and activate CAP to form the cAMP-CAP complex). This activates lac operon.