TCA

Question 1

Which is a prochiral molecule

Answer 1

Citrate

Question 2

Inhibitor of aconitase

Answer 2

Fluorocitrate

Question 3

Where does TCA occurs?

Answer 3

In mitochondria of eukaryotes and cytosol of prokaryotes.

Question 4

What step is substrate level phosphorylation

Answer 4

When succinyl CoA becomes Succinate and gives out ATP or GTP

Question 5

Which enzyme is present on the membrane?

Answer 5

Succinate dehydrogenase

Question 6

Name all the enzyme order wise

Answer 6

Citrate synthase
Aconitase
Isocitrate dehydrogenase
Alpha ketoglutarate dehydrogenase
Succinate thiokinase/ succinyl CoA synthase
Succinate Dehydrogenase
Fumerase
Malate dehydrogenase

Question 7

Process of conversion of succinyl CoA into Succinate

Answer 7

First, succinyl CoA reacts with P to form Succinyl phosphate and CoA.
Phosphoryl group transferred to His residue of enzyme nucleoside diphosphate kinase (NDK).
Phosphoryl group transferred to GDP or ADP.

Question 8

Inhibitor of succinate dehydrogenase

Answer 8

Malonate, a competitive inhibitor

Question 9

Inhibitor of alpha ketoglutarate dehydrogenase

Answer 9

Succinyl CoA and NADH

Question 10

Inhibitor of alpha ketoglutarate dehydrogenase

Answer 10

Succinyl CoA and NADH

Question 11

Total ATP formed

Answer 11

30 to 32 ATP

Question 12

Isocitrate dehydrogenase inhibitor and promoter

Answer 12

Inhibitor - NADH
Promotor - ADP

Question 13

Name the isomerisation step

Answer 13

Dehydration and Hydration

Question 14

What happens to the two carbon of acetyl CoA

Answer 14

Get oxidised into CO2 in both the oxidative decarboxylation step