Protein Flashcards
Aromatic amino acids
Tyrosine
Tryptophan
Phenylalanine
Most basic and most acidic protein
Basic - Arginine
Acidic - Aspartate
Amino acid per residues and pitch of alpha helix
3.6 amino acid per residues
Pitch - 0.54nm
Axial rise of alpha and beta protein
1.5 Å and 3.5 Å
Amino acid in Collegen
Glycine, proline and hydroproline/ hydroxylysine
Amino acid helps in hydroxylation of Collegen
Ascorbic acid
Amino acid of Elastin and Keratins
Glycine and Proline
Cysteine
Molecular chaperone and eg.
Proteins that help in interaction with other protein and aid in their folding.
Eg. Foldases and holdases.
Two subcomplex of proteasome
20S core and 19S proteasome forms 26 S or 30 S Proteasome.
Ubiquitin binds to protein by which bond and by which residue.
Covalent bond and by lysine residue
Ring of the following
Phenylalanine
Tryptophan
Cysteine
Histidine
Phenylalanine - Phenyl ring
Tryptophan - Indole ring
Cysteine - Sulfhydryl or thiol group
Histidine - Imidazole ring
Deamination of 5 methyl cytosine
Cytosine
Adenine
Guanine
Thymine
Uracil
Hypoxanthine
Xanthine
Degrons
Aka degradation signal guides the metabolic stability of proteins.
N degron
The first amino acid present in the n terminal that causes degradation
N end rule pathway
Proteins with degrons degrade protein by this pathway by ubiquitination
Reagents used to determine n terminal amino acid
1 fluoro 2,4 dinitrobenzene, Phenylisothiocyanate (Edman Reagent) and dansyl chloride
Method to determine sequence of amino acid of n terminal and name the chemical
Edman degradation method
Chemical - Phenylisothiocyanate (edman reagent)
Which amino acid is not found in its primary form and what is it called
Proline or imino acid is found in its secondary form only
What is isoelectric precipitation
Proteins and its isoelectric points are not soluble in water hence they get precipitated
Which bonds are responsible for tertiary structure
Hydrophobic bond (major bond)
Electostatic interaction or salt bridges or ionic bond
Hydrogen bond
Van der waal forces
What is a protein found in hemoglobin
Histidine
Which amino acid has its own trna
Selenocysteine
In a helix which helix is more favourable? Right or left?
Right handed
In a helix the intrachain bonds are formed between?
NH and CO
In b helix, which strand is more stable? Parallel and antiparallel
Antiparallel
Glycolsylation of protein occurs in?
ER and Golgi
Name of the milk protein
Casein
Which enzyme converts casein into paracasein
Renin
Name a natural antioxidant in mammals
Glutathione
Who detoxify NH3 in brain
Glutamine
Where is N End pathway present
Cytosol and nucleus
Name the reagents to determine N End Terminal
1 fluoro 2,4 dinitrobenzene
Dansyl chloride
Edman reagent
Name the reagent used to determine the sequence of amino acid
Edman Reagent or Phenylisothiocyanate
