Protein

Question 1

Aromatic amino acids

Answer 1

Tyrosine
Tryptophan
Phenylalanine

Question 2

Most basic and most acidic protein

Answer 2

Basic - Arginine
Acidic - Aspartate

Question 3

Amino acid per residues and pitch of alpha helix

Answer 3

3.6 amino acid per residues
Pitch - 0.54nm

Question 4

Axial rise of alpha and beta protein

Answer 4

1.5 Å and 3.5 Å

Question 5

Amino acid in Collegen

Answer 5

Glycine, proline and hydroproline/ hydroxylysine

Question 6

Amino acid helps in hydroxylation of Collegen

Answer 6

Ascorbic acid

Question 7

Amino acid of Elastin and Keratins

Answer 7

Glycine and Proline
Cysteine

Question 8

Molecular chaperone and eg.

Answer 8

Proteins that help in interaction with other protein and aid in their folding.
Eg. Foldases and holdases.

Question 9

Two subcomplex of proteasome

Answer 9

20S core and 19S proteasome forms 26 S or 30 S Proteasome.

Question 10

Ubiquitin binds to protein by which bond and by which residue.

Answer 10

Covalent bond and by lysine residue

Question 11

Ring of the following
Phenylalanine
Tryptophan
Cysteine
Histidine

Answer 11

Phenylalanine - Phenyl ring
Tryptophan - Indole ring
Cysteine - Sulfhydryl or thiol group
Histidine - Imidazole ring

Question 12

Deamination of 5 methyl cytosine
Cytosine
Adenine
Guanine

Answer 12

Thymine
Uracil
Hypoxanthine
Xanthine

Question 13

Degrons

Answer 13

Aka degradation signal guides the metabolic stability of proteins.

Question 14

N degron

Answer 14

The first amino acid present in the n terminal that causes degradation

Question 15

N end rule pathway

Answer 15

Proteins with degrons degrade protein by this pathway by ubiquitination

Question 16

Reagents used to determine n terminal amino acid

Answer 16

1 fluoro 2,4 dinitrobenzene, Phenylisothiocyanate (Edman Reagent) and dansyl chloride

Question 17

Method to determine sequence of amino acid of n terminal and name the chemical

Answer 17

Edman degradation method
Chemical - Phenylisothiocyanate (edman reagent)

Question 18

Which amino acid is not found in its primary form and what is it called

Answer 18

Proline or imino acid is found in its secondary form only

Question 19

What is isoelectric precipitation

Answer 19

Proteins and its isoelectric points are not soluble in water hence they get precipitated

Question 20

Which bonds are responsible for tertiary structure

Answer 20

Hydrophobic bond (major bond)
Electostatic interaction or salt bridges or ionic bond
Hydrogen bond
Van der waal forces

Question 21

What is a protein found in hemoglobin

Answer 21

Histidine

Question 22

Which amino acid has its own trna

Answer 22

Selenocysteine

Question 23

In a helix which helix is more favourable? Right or left?

Answer 23

Right handed

Question 24

In a helix the intrachain bonds are formed between?

Answer 24

NH and CO

Question 25

In b helix, which strand is more stable? Parallel and antiparallel

Answer 25

Antiparallel

Question 26

Glycolsylation of protein occurs in?

Answer 26

ER and Golgi

Question 27

Name of the milk protein

Answer 27

Casein

Question 28

Which enzyme converts casein into paracasein

Answer 28

Renin

Question 29

Name a natural antioxidant in mammals

Answer 29

Glutathione

Question 30

Who detoxify NH3 in brain

Answer 30

Glutamine

Question 31

Where is N End pathway present

Answer 31

Cytosol and nucleus

Question 32

Name the reagents to determine N End Terminal

Answer 32

1 fluoro 2,4 dinitrobenzene
Dansyl chloride
Edman reagent

Question 33

Name the reagent used to determine the sequence of amino acid

Answer 33

Edman Reagent or Phenylisothiocyanate