Protein Flashcards

1
Q

Aromatic amino acids

A

Tyrosine
Tryptophan
Phenylalanine

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2
Q

Most basic and most acidic protein

A

Basic - Arginine
Acidic - Aspartate

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3
Q

Amino acid per residues and pitch of alpha helix

A

3.6 amino acid per residues
Pitch - 0.54nm

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4
Q

Axial rise of alpha and beta protein

A

1.5 Å and 3.5 Å

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5
Q

Amino acid in Collegen

A

Glycine, proline and hydroproline/ hydroxylysine

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6
Q

Amino acid helps in hydroxylation of Collegen

A

Ascorbic acid

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7
Q

Amino acid of Elastin and Keratins

A

Glycine and Proline
Cysteine

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8
Q

Molecular chaperone and eg.

A

Proteins that help in interaction with other protein and aid in their folding.
Eg. Foldases and holdases.

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9
Q

Two subcomplex of proteasome

A

20S core and 19S proteasome forms 26 S or 30 S Proteasome.

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10
Q

Ubiquitin binds to protein by which bond and by which residue.

A

Covalent bond and by lysine residue

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11
Q

Ring of the following
Phenylalanine
Tryptophan
Cysteine
Histidine

A

Phenylalanine - Phenyl ring
Tryptophan - Indole ring
Cysteine - Sulfhydryl or thiol group
Histidine - Imidazole ring

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12
Q

Deamination of 5 methyl cytosine
Cytosine
Adenine
Guanine

A

Thymine
Uracil
Hypoxanthine
Xanthine

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13
Q

Degrons

A

Aka degradation signal guides the metabolic stability of proteins.

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14
Q

N degron

A

The first amino acid present in the n terminal that causes degradation

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15
Q

N end rule pathway

A

Proteins with degrons degrade protein by this pathway by ubiquitination

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16
Q

Reagents used to determine n terminal amino acid

A

1 fluoro 2,4 dinitrobenzene, Phenylisothiocyanate (Edman Reagent) and dansyl chloride

17
Q

Method to determine sequence of amino acid of n terminal and name the chemical

A

Edman degradation method
Chemical - Phenylisothiocyanate (edman reagent)

18
Q

Which amino acid is not found in its primary form and what is it called

A

Proline or imino acid is found in its secondary form only

19
Q

What is isoelectric precipitation

A

Proteins and its isoelectric points are not soluble in water hence they get precipitated

20
Q

Which bonds are responsible for tertiary structure

A

Hydrophobic bond (major bond)
Electostatic interaction or salt bridges or ionic bond
Hydrogen bond
Van der waal forces

21
Q

What is a protein found in hemoglobin

22
Q

Which amino acid has its own trna

A

Selenocysteine

23
Q

In a helix which helix is more favourable? Right or left?

A

Right handed

24
Q

In a helix the intrachain bonds are formed between?

25
In b helix, which strand is more stable? Parallel and antiparallel
Antiparallel
26
Glycolsylation of protein occurs in?
ER and Golgi
27
Name of the milk protein
Casein
28
Which enzyme converts casein into paracasein
Renin
29
Name a natural antioxidant in mammals
Glutathione
30
Who detoxify NH3 in brain
Glutamine
31
Where is N End pathway present
Cytosol and nucleus
32
Name the reagents to determine N End Terminal
1 fluoro 2,4 dinitrobenzene Dansyl chloride Edman reagent
33
Name the reagent used to determine the sequence of amino acid
Edman Reagent or Phenylisothiocyanate