Oxidative Phosphorylation

Question 1

Name all the four respiratory enzymes

Answer 1

NADH - Coenzyme Q reductase or NADH dehydrogenase
FADH2 - Coenzyme Q reductase
Coenzyme Q - Cytochrome C reductase or cytochrome bc1 complex
Cytochrome C oxidase

Question 2

Where does oxidative phosphorylation takes place

Answer 2

Inner mitochondrial membrane
Prokaryotes - plasma membrane

Question 3

What is respirasome

Answer 3

Complex 1, 2 and 3 appear to be associated in a supramolecular complex termed respirasome.

Question 4

Prosthetic group of complex

Answer 4

I - FMN and Fe- S
II - FAD, Fe - S
III - Heme, Fe - S
IV - Heme, Cu

Question 5

Subunits of complex

Answer 5

I 46
II 4
III 11
IV 13

Question 6

Subunits of complex

Answer 6

I 46
II 4
III 11
IV 13

Question 7

Does reduction potential increases as we move along the complex

Answer 7

Yes

Question 8

Name all the three redox stages of Coenzyme Q

Answer 8

Ubiquinone (fully oxidised)
Semiubiquinone (partially reduced)
Ubiquinol (reduced)

Question 9

Which complex does not pump protons

Answer 9

Complex 2

Question 10

Process of Q cycle

Answer 10

First the one electron will go to Fe-S to cyt C. The other to low affinity cyt b the to high affinity cyt b. Forms semiquinone. Cycle repeats and forms quinol.

Question 11

How many heme group and copper ions does complex four have

Answer 11

2 heme and 3 copper ions

Question 12

What is the active centre of oxygen

Answer 12

Cyt a3 Cu b

Question 13

How many electrons transfer to oxygen

Answer 13

4 electrons to form 2 water molecules

Question 14

Name all the inhibitors of all the complexes

Answer 14

Rotenone, amobarbital, piericidin - I
Antimycin A - III
Cyanide, azide and CO - IV (Cyt a3)

Question 15

Name all the inhibitors of all the complexes

Answer 15

Rotenone, amobarbital, piericidin - I
Antimycin A - III
Cyanide, azide and CO - IV (Cyt a3)

Question 16

What is the alternative pathway for electron flow

Answer 16

Cyanide Resistant Pathway

Question 17

Where is the pH higher in the mitochondria

Answer 17

Matrix

Question 18

Which side of the mitochondria is negative

Answer 18

Matrix

Question 19

What is the electric potential difference and pH gradient difference in the mitochondria

Answer 19

Around 160mV
And 1 unit pH

Question 20

Two components of ATP synthase/F1F° complex/ Complex V

Answer 20

F° has 1 a subunit, 2 b subunit and 9 - 12 c subunit. An aspartic acid also lies within them
F1 has 3 alpha, 3 beta, gamma, delta and epsilon subunit.

Question 21

Two components of ATP synthase/F1F° complex/ Complex V

Answer 21

F° has 1 a subunit, 2 b subunit and 9 - 12 c subunit. An aspartic acid also lies within them
F1 has 3 alpha, 3 beta, gamma, delta and epsilon subunit.

Question 22

Unit comprises of stator and rotor

Answer 22

Stator - a, b2 and alpha3, beta 3 and delta
Rotor - C9 - 12, gamma and epsilon

Question 23

Which complex act as a reversible coupling agent

Answer 23

F1-ATPase

Question 24

Paul Boyer gave what?

Answer 24

Binding change mechanism /Flip-flop mechanism

Question 25

How many H+ flow in to produce ATP

Answer 25

4

Question 26

Name uncoupling agent

Answer 26

DNP, Dicoumarol and FCCP, Thermogenin and Valinomycin

Question 27

What is ATP - ADP Translocase

Answer 27

Is an antiporter which gives matrix a negative charge.

Question 28

Who inhibits ATP - ADP Translocase

Answer 28

Atractiloside

Question 29

What is ATP Synthasome

Answer 29

Phosphate Translocase and ADP - ATP Translocase

Question 30

Lactic acid cycle is also known as?

Answer 30

Cori cycle

Question 31

Pasteur Effect

Answer 31

Glucose and ethanol consumption drop in the presence of Oxygen

Question 32

Warburg Effect

Answer 32

Lactic acid fermentation in the presence of Oxygen