Oxidative Phosphorylation Flashcards
Name all the four respiratory enzymes
NADH - Coenzyme Q reductase or NADH dehydrogenase
FADH2 - Coenzyme Q reductase
Coenzyme Q - Cytochrome C reductase or cytochrome bc1 complex
Cytochrome C oxidase
Where does oxidative phosphorylation takes place
Inner mitochondrial membrane
Prokaryotes - plasma membrane
What is respirasome
Complex 1, 2 and 3 appear to be associated in a supramolecular complex termed respirasome.
Prosthetic group of complex
I - FMN and Fe- S
II - FAD, Fe - S
III - Heme, Fe - S
IV - Heme, Cu
Subunits of complex
I 46
II 4
III 11
IV 13
Subunits of complex
I 46
II 4
III 11
IV 13
Does reduction potential increases as we move along the complex
Yes
Name all the three redox stages of Coenzyme Q
Ubiquinone (fully oxidised)
Semiubiquinone (partially reduced)
Ubiquinol (reduced)
Which complex does not pump protons
Complex 2
Process of Q cycle
First the one electron will go to Fe-S to cyt C. The other to low affinity cyt b the to high affinity cyt b. Forms semiquinone. Cycle repeats and forms quinol.
How many heme group and copper ions does complex four have
2 heme and 3 copper ions
What is the active centre of oxygen
Cyt a3 Cu b
How many electrons transfer to oxygen
4 electrons to form 2 water molecules
Name all the inhibitors of all the complexes
Rotenone, amobarbital, piericidin - I
Antimycin A - III
Cyanide, azide and CO - IV (Cyt a3)
Name all the inhibitors of all the complexes
Rotenone, amobarbital, piericidin - I
Antimycin A - III
Cyanide, azide and CO - IV (Cyt a3)
What is the alternative pathway for electron flow
Cyanide Resistant Pathway
Where is the pH higher in the mitochondria
Matrix
Which side of the mitochondria is negative
Matrix
What is the electric potential difference and pH gradient difference in the mitochondria
Around 160mV
And 1 unit pH
Two components of ATP synthase/F1F° complex/ Complex V
F° has 1 a subunit, 2 b subunit and 9 - 12 c subunit. An aspartic acid also lies within them
F1 has 3 alpha, 3 beta, gamma, delta and epsilon subunit.
Two components of ATP synthase/F1F° complex/ Complex V
F° has 1 a subunit, 2 b subunit and 9 - 12 c subunit. An aspartic acid also lies within them
F1 has 3 alpha, 3 beta, gamma, delta and epsilon subunit.
Unit comprises of stator and rotor
Stator - a, b2 and alpha3, beta 3 and delta
Rotor - C9 - 12, gamma and epsilon
Which complex act as a reversible coupling agent
F1-ATPase
Paul Boyer gave what?
Binding change mechanism /Flip-flop mechanism
