T3M1 Flashcards
Genes are made up of
DNA
Genes contain the instructions to make
proteins
Genes can vary in size from
few hundred DNA nucleotide bases to thousands
Human genome project estimates humans have how many genes?
20,000- 25,000 genes that code for proteins (some coding for different versions of the same protein)
Housekeeping genes are constitutively expressed
‘ON’ all the time includes: structural proteins, ribosomal
proteins etc.
Regulated genes are expressed
only when needed (enzymes)
Fastest way to express a gene
post-translational regulation
Slowest way to express a gene
transcriptional control regulation
Most efficient way to express a gene
transcriptional regulation
Transcriptional Control
Ability of RNA polymerase to bind to or transcribe a gene
Translational Control
Translation rates, lifetime of RNA
Post-translational Control
Post-translational modifications which may change folding and/or activity
What is needed for prokaryotic growth?
favourable temperature, nutrient rich environment with amino acids and carbohydrates
The DNA of the bacterial nucleoid contains
the information that is required to orchestrate a
response to any change in the environment
Housekeeping genes
genes that are required all of the time for normal functions
Regulated genes
genes can be turned on and off on an as-needed basis
What type of gene is constitutively
expressed, and always being transcribed and translated?
housekeeping genes
Housekeeping gene examples
genes that are important for structural proteins of the cell, RNA and DNA polymerases and genes that are coding for ribosomal proteins
Housekeeping genes allow for
constant maintenance of general cellular activities
When exposed to a changing environment, bacterial cells can respond by
altering the expression pattern of some genes