Structure of Genome Part 4 Flashcards
1
Q
the relationship between size and complexity in genomes
A
- in genomes with repetitive DNA, the sequence of each type of repeat adds to the complexity only once, no matter how often it is repeated.
2
Q
why Cot curves containing various components of repetitive DNA have several phases.
A
- repetitive DNA reassociates first because it’s more concentrated
- then lag phase
- then unique DNA will reassociate
3
Q
which reassociates faster
A
- repetitive DNA because it is less complex
4
Q
why plot Cot
A
- experiments using a single concentration of DNA would take years
5
Q
calculating the complexity
A
Co1/2 (genome or portion of genome) / Cot 1/2 E. coli DNA
= complexity of any genome or portion of genome/ complexity of E. coli DNA (4.2*10^6)
