DNA sequencing comparison Flashcards
1
Q
Types of sequencing
A
- Sanger-automated
- Pyrosequencing
- Illumina
- Ion Torrent
- Pac Bio
- Nanopore
2
Q
genomic library cloned into plasmids
A
- Sanger automated
3
Q
uses chain termination
A
- Sanger automated
- Illumina
4
Q
uses DNA synthesis
A
- Sanger-automated
- Pyrosequencing
- Illumina
- Ion Torrent
- Pac Bio
5
Q
Requires a genomic library
A
- Sanger-automated
- Pyrosequencing
- Illumina
- Ion Torrent
- Pac Bio
6
Q
involves massively parallel sequencing
A
- pyrosequencing
- illumina
- ion torrent
- pac bio
- nanopore
7
Q
measures the release of pyrophosphate
A
- pyrosequencing
8
Q
measures light production
A
- pyrosequencing
9
Q
is the most expensive to sequence an entire genome
A
- pyrosequencing
10
Q
best for highly repetitive DNA
A
- Pac Bio
11
Q
measures the release of H+ ions
A
- Ion torrent
12
Q
generates paired end reads
A
- Illumina
13
Q
most likely used in the clinic
A
- nanopore
14
Q
used for personal sequencing
A
- Pyrosequencing
15
Q
real time single molecule sequencing via movie production
A
- Pac Bio
