Repetitive DNA Part 2 Flashcards

1
Q

2 types of analytical ultracentrifugation

A
  • equilibrium or bouyant density centrifugation

- sedimentation velocity centrifugation

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2
Q

two types of ultracentrifugation

A
  • analytical

- preparative

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3
Q

analytical

A
  • used to study the properties of molecules
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4
Q

preparative

A
  • used to purify molecules by separating from other molecules so that their properties can be studied by other means
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5
Q

Equilibrium or bouyant density centrifugation

A
  • high molecular weight DNA sheared and mixed with CsCl and a subjected to high g forces
  • CsCl forms a gradient from highest density at the bottom to lowest density at the top
  • DNA migrates to a position in the gradient where the density matches the density of the CsCl
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6
Q

what is formed in Equilibrium or bouyant density centrifugation

A
  • a broad band is formed covering a range of fragments of different densities
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7
Q

Bouyant density is a function of

A
  • G+C content

- not size

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8
Q

Higher G+C content

A
  • higher density
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9
Q

if the satellite DNA has a GC content nearly the same as main GC content

A
  • no satellite would be visible at all
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10
Q

sedimentation validity is a function of

A
  • density
  • size
  • shape
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11
Q

how sedimentation velocity works

A
  • put molecules on top of solution in centrifuge tube

- turn on centrifuge and measure rate of migration down the tube

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12
Q

large molecules sediment

A
  • faster
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13
Q

what makes things sediment faster

A
  • compact state
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14
Q

what makes things sediment slower

A
  • extended state
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15
Q

How repetitive DNA can sometimes (but not always) be recognized as satellite DNA

A
  • because of GC content that is different from the overall average GC content of DNA in the genome.
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16
Q

S values in complexes with subunits

A
  • the S values of the subunits are not expected to add up to the S value of the entire complex.