STAINS AND INTERPRETATIONS

Question 1

The identification of these enzyme in the cytoplasm of the leukocytes is useful in distinguishing** acute
lymphocytic leukemia from acute myelogenous leukemia.**
⮚ Positive reaction goes yellow-brown stained granules in the cytoplasm of the cell

A. Myeloperoxidase (MPX)
B.Sudan Black B
C. Esterases
D.Periodic–acid-schif

Answer 1

A. Myeloperoxidase (MPX)

Question 2

ARE PEROXIDASE NEGATIVE

A. NEUTROPHIL
B.EOSINOPHIL
C. GRANULOCYTE
D. MONOBLAST

Answer 2

D. MONOBLAST

Question 3

**MYELOCYTE IN THE CENTER STAINS PEROXIDASE **
A. POSITIVE
B NEGATIVE

Answer 3

A. POSITIVE

Question 4

INHIBITS THE PEROXIDASE REACTION

A. EDTA
B. SERUM
C. SST
D.CALCIUM

Answer 4

A. EDTA

Question 5

This stain is specific for lipids.

A. Myeloperoxidase (MPX)
B.Sudan Black B
C. Esterases
D.Periodic–acid-schif

Answer 5

B.Sudan Black B

Question 6

neutral fat, phospholipids and sterols.

A. Myeloperoxidase (MPX)
B.Sudan Black B
C. Esterases
D.Periodic–acid-schif

Answer 6

B.Sudan Black B

Question 7

** IS SIMILAR TO THE PEROXIDASE REACTION IN
DISTINGUISHING ACUTE LYMPHOCYTIC LEUKEMIA FROM ACUTE MYELOGENOUS LEUKEMIA**

A. SUDAN BLACK B STAIN
B.Esterases STAIN

Answer 7

A. SUDAN BLACK B STAIN

Question 8

Possibly a little more sensitive for the early myeloid cells.
A. SUDAN BLACK B STAIN
B.Esterases STAIN

Answer 8

A. SUDAN BLACK B STAIN

Question 9

using different substrates allows the distinction between cells of the monocyte lineage and the cells of the neutrophil lineage.

A. SUDAN BLACK B STAIN
B.Esterases STAIN

Answer 9

B.Esterases STAIN

Question 10

most suitable identifying monoblastic types of leukemia

A.a-napthyl acetate
B.1-naphthyl-acetate-esterase

Answer 10

B.1-naphthyl-acetate-esterase

Question 11

Esterases

Released naphthol rapidly couples with a _ present in the staining mixture, resulting in a
brightly **red-brown or black-brown **precipitate at or near the site enzymatic activity.

A. diazonium salt
B.naphtyl butyrate

Answer 11

A. diazonium salt

Question 12

Two substrate esters commonly used which is non-specific

Answer 12

1. a-napthyl acetate
2. a-naphtyl butyrate

Question 13

specific

a.a-Naphthyl AS-d chloroacetate esterase
b. -Naphthyl AS-t chloraacete

Answer 13

a.a-Naphthyl AS-d chloroacetate esteras

Question 14

the cytoplasm of polymorphonuclear leukocytes stains intensely pink or red, with granular appearance in some cells.

This reaction stains polysaccharides.
a. periodic–acid-schiff
b. Leukocyte Alkaline Phosphatase

Answer 14

a. periodic–acid-schiff

Question 15

⮚ Enzyme activity is useful for differentiating chronic myelogenous leukemia from a leukemoid reaction that may be seen in severe infections.

a. Leukocyte Alkaline Phosphatase
b.a. periodic–acid-schiff

Answer 15

a. Leukocyte Alkaline Phosphatase

Question 16

indicated by red granular precipitate and is demonstrable in most cells of the
hematopoietic system

a. Leukocyte Alkaline Phosphatase
b.a. periodic–acid-schiff
c. Acid Phosphatase

Answer 16

c. Acid Phosphatase

Question 17

Very little acid phosphatase activity is present in normal

a. lymphocytes and erythroblasts
b. lymphocytes and monoblast

Answer 17

a. lymphocytes and erythroblasts

Question 18

Increased acid phosphatase activity is observed in abnormal mononuclear cells of patients with

Answer 18

hairy cell leukemia

Question 19

Useful in diagnosing hematologic malignancies

Answer 19

flow cytometry

Question 20

**FLOW CYTOMETRY 3 spx **

Answer 20

bone marrow, peripheral blood, and lymphoid tissue

Question 21

FLOW CYTOMETRY

Peripheral blood and bone marrow specimens should be processed within _ hours from their collection

a.24 to 48
b 24-to 32

Answer 21

a.24 to 48

Question 22

FLOW CYTOMETRY

**To test viability, trypan blue exclusion or flow cytometry of a specimen stained with **

a.propidium iodide
b sodium iodide

Answer 22

a.propidium iodide