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A. GPHCLAB > STAINING > Flashcards

STAINING Flashcards

1
Q

Iron hematoxylin

A
  • Weigert’s stain with Ferric chloride
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2
Q

Active colorinh agent in hematoxylin

A

Hematin

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3
Q

Alum hematoxylin stains (2)

A
  • ehrlich’s soln w/ Na iodate

- harris’ soln with Mercuric chloride

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4
Q

Hematoxylin : SN mexican tree

A

Hematoxylin Campechianum

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6
Q

Oxidizing agents for hematoxylin

A
  • H202
  • Mercuric oxide
  • Na iodate
  • Na perborate
  • K permanganate
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7
Q

SN female cochineal bug

A

Coccus cacti

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8
Q

Powerful chromatin and nuclear stain

A

Carmine dye

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9
Q

Stains elastic fibers

A

Orcein

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10
Q

Composition of orcein

A

vegetable dye (lichen) + ammonia (blue/violet)

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11
Q

Biological stains

A

Natural dyes || Synthetic Dyes

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12
Q

Oxidation process of hematin

A

Ripening

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14
Q

dye classification (3)

A

acid dye
basic dye
neutral dye

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15
Q

Carmine dye for Neuropathological studies

A

Picrocarmine

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16
Q

Coal tar is derived from?

A

Hydrocarbon benzene

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17
Q

Staining Reaction based on: (4)

A

1) Capillary osmosis
2) Solubility
3) Adsorption
4) Absorption

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17
Q

Mordant used in hematoxylin

A
  • Alum
  • Iron
  • Copper
  • Chromium
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18
Q

Spermatogenesis study

A

Copper hematoxylin

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18
Q

Carmine dye with aluminum chloride for demonstration of GLYCOGEN

A

Best Carmine stain

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19
Q

Fixation of basic dyes

A
  • M. chloride

- Formaldehyde

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20
Q

coal tar dyes

A

synthetic dyes

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21
Q

Fxn of acid dyes

A
  • chromium
  • picric acid
  • trichloroacetic acid
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22
Q

regressive staining, diff. w/ 1% HCl in 70% OH

A

Ehrlich’s hematoxylin

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23
Q

a basic dye which serves as an indicator and dye

A

methylene blue

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25
Q

Routine nuclear staining, exfoliative cytology, sex chromosomes

A

Harris hematoxylin

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26
Q

iron hematoxylin that demonstrates muscle fibers and connective tissue

A

weigert’s

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27
Q

auxilliary radical added to retain color

A

auxochrome

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27
Q

fiserves as a fixative, decalcifying agent, and a softener

A

acid dyes

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28
Q

neutral dye - leukocyte differentiation (2)

A
  • giemsa stain

- leishman stain

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29
Q

iron hematoxylin for cytological stain regressive staining of thin sections

A

heidenhain’s

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30
Q

routine counterstain, background contrasting stain

A

eosin

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31
Q

Celestine blue hemalum method of nuclear staining

A

Mayer hematoxylin

32
Q

stains amyloid and platelets

A

crystal violet

33
Q

stains leukocyte nuclei

A

methylene violet

34
Q

counterstaining of epithelial sections

A

aniline blue

35
Q

nissl granules and chromophilic bodies

A

toluidine blue

36
Q

vital stain for circulatory system

A

prussian blue

37
Q

method used in staining elastic fibers using orcein

A

Taenzer unna orcein method

38
Q

stains parasite and bacteria

A

malachite green

40
Q

for blood smear

A

giemsa stain

41
Q

stains acid fast organism

A

basic fuchsin

41
Q

counterstain in papanicolaou method

A

bismarck brown

42
Q

stain for acid mucopolysaccharides

A

alcian blue

43
Q

embryos, elastic tissue, amyloid, and myelin

44
Q

specific for connective tissue and epithelial mucin

A

alcian blue

45
Q

cell granules and vacuoles of phagocytic cells

A

neutral red

46
Q

neuroglia (frozen section)

A

victoria blue

47
Q

discrimination of dead and living cells

A

acridine orange

48
Q

calcium deposits, sites phosphate activities

A

acridine red

49
Q

Method in embryos using congo red

A

Kraijan’s method

51
Q

intravital staining of mitochondria

A

janus green B

51
Q

substitute for carbol fuchsin

A

night blue

52
Q

fat stains

A

lysochrome

53
Q

for hemoglobin

54
Q

spirochete, reticulum and other fibers

A

silver nitrate

55
Q

griesbach method with osmic acid, blood, glandular tissue

A

rhodamine b

56
Q

stain solvents (4)

A
  • distilled H20
  • alcohol
  • aniline water
  • phenol
57
Q

fixative and fat stain in frozen section

A

osmium tetroxide

58
Q

stains phospholipids, TAG

A

sudan black

60
Q

using simple stain with aqueous or OH solution of dye

A

Direct staining

61
Q

stains TAG and no reaction to phospholipids and fine lipid

61
Q

stains CNS fats

62
Q

staining with time sequence and no washing/decolorization

A

Progressive staining

63
Q

stain with mordant

A

Indirect staining

64
Q

accelerate or speed up staining reaction

A

accentuator

65
Q

overstaining and then decolorization

A

Regressive staining

66
Q

stain color reaction different from the color of ten stain itself

A

metachromasia

67
Q

using a different stain for contrast and background

A

counterstaining

68
Q

general differentiation of nucleus and cytoplasm

A

microanatomical staining

69
Q

using colorless soln of metallic salts

A

metallic impregnation

70
Q

selective staining of living cells

A

vital staining

71
Q

stain for RES

A

Trypan blue

72
Q

Best vital dye

A

neutral red

73
Q

injecting the dye before extraction of the tissue

A

intravital staining

74
Q

staining immediately after removal of the tissue from the body

A

supravital staining

75
Q

Variation in affinities for most dyes and stains

A

Differentiation

A. GPHCLAB (12 decks)

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