Adhesives-Mounting media

Question 1

ways to removed bubbles (2)

Answer 1

• using smooth teasing needle

- pulling the ribbon very gently across the long edge of a glass slide held below the section in the water bath

Question 2

ways to fixed the sections in the slide (3)

Answer 2

1) leaving the slides in a 37°C incubator overnight
2) placing the slides in a wax oven at 56-60°C for 2 hrs
3) drying the slides on a hot plate at 45-55°C for 30-45 mins

Question 3

Use an alternative for drying

Answer 3

adhesives

Question 4

essential for methods that require exposure of sections to acids and alkalis during staining

Answer 4

Adhesives

Question 5

8 common adhesives used

Answer 5

1) mayer’s egg albumin
2) dried albumin
3) gelatin (1%)
4) gelatin-formaldehyde mixture
5) starch paste
6) Plasma
7) Poly-L-Lysine
8) APES (3-aminopropylthriethoxysilane)

Question 6

formula of mayer’s egg albumin

Answer 6

• egg white (50 cc)

- glycerin (50 cc)

Question 7

Most commonly used adhesives because it very easy to make

Answer 7

mayer’s egg albumin

Question 8

prevent the growth of mold

Answer 8

100 mg. thymol crystals

Question 9

formula of dried albumin

Answer 9

• dried albumin (5gms)

- NaCl (5gms)

Question 10

formula of gelatin

Answer 10

• gelatin (1 gm)
• dist. H2O (100 ml)
• glycerol (15 ml)
• phenol crystals (2 gm)

Question 11

formula of gelatin-formaldehyde

Answer 11

• 1% gelatin (5 ml)

- 2% formaldehyde (5 ml)

Question 12

formula of starch paste

Answer 12

• powdered starch (1 gm)
• dist. H20 (30 ml)
• N/I HCl (2 drops)

Question 13

readily available from outdated blood stored in blood banks, dispensed into sterile tubes of .5 ml each

Answer 13

plasma

Question 14

an aqueous detergent that is widely used as a section adhesive in immunohistochemistry

Answer 14

poly-L-lysine

Question 15

useful in cytology, particularly for cytospin prep of proteinacious or bloody material

Answer 15

APES

Question 16

usually a syrupy fluid applied between the section and the coverslip after staining, it preserves the slides for permanent keeping, facilitate easy handling and storage

Answer 16

mounting medium

Question 17

prevent the distortion of image during microscopic examination

Answer 17

mounting mediumin

Question 18

incubation temp and period after mounting to harden the medium

Answer 18

37°C for 12-24 hrs

Question 19

characteristic of a good mounting medium

Answer 19

• refractive index of the mountant should be as near as possible to that of the glass which is 1.518
• miscible w/ xylene and toluene
• it should not dry quickly

Question 20

two main groups of mounting media

Answer 20

• aqueous media

- resinous media

Question 21

designed to mount water-miscible prep directly from water in cases of?

Answer 21

• aqueous media

in cases of?

• the stain is removed or decolorized with alc. or xylene
• for metachromatic staining of amyloid

Question 22

solidify the mounting medium

Answer 22

gum arabic

Question 23

prevent cracking and drying of the prep

Answer 23

glycerol

Question 24

increase the refractive index

Answer 24

sugar

Question 25

examples of common aqueous mounting media (5)

Answer 25

1) water
2) glycerin
3) farrant’s medium
4) apathy’s medium
5) brun’s fluid

Question 26

used for prep that have been dehydrated and cleared in xylene or toluene, and are recommended for majority of staining methods

Answer 26

resinous media

Question 27

refractive index of Canada balsam

Answer 27

1.524

Question 28

refractive index of DPX

Answer 28

1.532

Question 29

refractive index of XAM

Answer 29

1.52

Question 30

refractive index of CLARITE

Answer 30

1.544

Question 31

the process of sealing the margins of the cover slip

Answer 31

ringing