Adhesives-Mounting media Flashcards
ways to removed bubbles (2)
- using smooth teasing needle
- pulling the ribbon very gently across the long edge of a glass slide held below the section in the water bath
ways to fixed the sections in the slide (3)
1) leaving the slides in a 37°C incubator overnight
2) placing the slides in a wax oven at 56-60°C for 2 hrs
3) drying the slides on a hot plate at 45-55°C for 30-45 mins
Use an alternative for drying
adhesives
essential for methods that require exposure of sections to acids and alkalis during staining
Adhesives
8 common adhesives used
1) mayer’s egg albumin
2) dried albumin
3) gelatin (1%)
4) gelatin-formaldehyde mixture
5) starch paste
6) Plasma
7) Poly-L-Lysine
8) APES (3-aminopropylthriethoxysilane)
formula of mayer’s egg albumin
- egg white (50 cc)
- glycerin (50 cc)
Most commonly used adhesives because it very easy to make
mayer’s egg albumin
prevent the growth of mold
100 mg. thymol crystals
formula of dried albumin
- dried albumin (5gms)
- NaCl (5gms)
formula of gelatin
- gelatin (1 gm)
- dist. H2O (100 ml)
- glycerol (15 ml)
- phenol crystals (2 gm)
formula of gelatin-formaldehyde
- 1% gelatin (5 ml)
- 2% formaldehyde (5 ml)
formula of starch paste
- powdered starch (1 gm)
- dist. H20 (30 ml)
- N/I HCl (2 drops)
readily available from outdated blood stored in blood banks, dispensed into sterile tubes of .5 ml each
plasma
an aqueous detergent that is widely used as a section adhesive in immunohistochemistry
poly-L-lysine
useful in cytology, particularly for cytospin prep of proteinacious or bloody material
APES
usually a syrupy fluid applied between the section and the coverslip after staining, it preserves the slides for permanent keeping, facilitate easy handling and storage
mounting medium
prevent the distortion of image during microscopic examination
mounting mediumin
incubation temp and period after mounting to harden the medium
37°C for 12-24 hrs
characteristic of a good mounting medium
- refractive index of the mountant should be as near as possible to that of the glass which is 1.518
- miscible w/ xylene and toluene
- it should not dry quickly
two main groups of mounting media
- aqueous media
- resinous media
designed to mount water-miscible prep directly from water in cases of?
- aqueous media
in cases of?
- the stain is removed or decolorized with alc. or xylene
- for metachromatic staining of amyloid
solidify the mounting medium
gum arabic
prevent cracking and drying of the prep
glycerol
increase the refractive index
sugar
examples of common aqueous mounting media (5)
1) water
2) glycerin
3) farrant’s medium
4) apathy’s medium
5) brun’s fluid
used for prep that have been dehydrated and cleared in xylene or toluene, and are recommended for majority of staining methods
resinous media
refractive index of Canada balsam
1.524
refractive index of DPX
1.532
refractive index of XAM
1.52
refractive index of CLARITE
1.544
the process of sealing the margins of the cover slip
ringing
