Dehydration-clearing

Question 1

GENERAL RULE: whatever dehydrating agent is used, the vol should be?

Answer 1

not ljess than 10x the vol of tissue

Question 2

utilized in plant and animal micro-techniques

Answer 2

butyl alc

Question 3

additive to dehydrating agents

Answer 3

4% phenol

Question 3

clearing agent recommeded for CNS and cytological studies (smooth muscles and skin)

Answer 3

cedarwood oil

Question 4

less shrinkage and hardening than ethanol

Answer 4

butyl alc

Question 5

temp which will harden dehydration?

to insure complete dehydration?

Answer 5

37 C

layer of anhydrous CuSO4 (1/4 inch deep) covered w/ filter paper

Question 6

slow acting clearing agents used when double embedding techniques are required (2)

Answer 6

methyl benzoate

methyl salicylate

Question 7

commonly used dehydrating agents (5)

Answer 7

alcohol
acetone
dioxane-4-cellosolve
triethyl phosphate
tetrahydrofuran

Question 8

special clearing agents (2)

Answer 8

glycerin

gum syrup

Question 8

tissue placed in chloroform become translucent, T/F?

Answer 8

F

Question 10

process of removing inter/extracellular water from the tissue next to fixation and prior to wax impregantion is known as

Answer 10

dehydration

Question 11

alc for blood and tissue films and for smear prep

Answer 11

methanol

Question 15

acetone dehydrates for how long

Answer 15

1/2-2 hrs

Question 16

can use to store tissue for months w/o producing hardening or distortion?
combustible at what temp?

Answer 16

cellosolve

110-120 F

Question 17

dehydation of embryonic tissues starting from

Answer 17

30% ethanol

Question 18

process wherein alc or dehydrating agents are removed

Answer 18

clearing

Question 19

most commonly used clearing agent?
clearing time?
tissue block size?

Answer 19

xylene/xylol

1/2-1 h

Question 20

most commonly used clearing agents (4)

Answer 20

xylene
dioxane
chloroform
cedarwood

Question 21

triethyl phosphate is soluble in (7)

Answer 21

water
ether
alcohol
benzene
chloroform
acetone
xylene

Question 22

substitute for xylene or benzene?

time recommended?

Answer 22

toluene

1-2 hrs

Question 23

clearing agent recommended for urgent biopsies?

time?

Answer 23

benzene

15-60 mins

Question 24

slower than xylene but causes less brittleness?

thickness up to ___ can be processed?

Answer 24

chloroform

1 cm

Question 25

recommeded for clearing embryos, insects and delicate specimens

Answer 25

aniline oil

Question 26

alc recommnded for routing dehydation of tissue

Answer 26

ethanol

Question 27

used to clear both paraffin and celloidin sections during embedding process?
clearing time?

Answer 27

cedarwood oil

2-3 days

Question 28

properties are very similiar to chloroform

Answer 28

CCl4

Question 35

substance which absorbs the water from the tissue in weiseberger’s method

Answer 35

Ca oxide

Question 40

2 methods in dioxane?

Answer 40

graupner’s method

weiseberger’s method