Embedding

Question 1

Placing the tissue specimen in a precise position in a mold together with the labeled paper strip

Answer 1

Embedding

Question 2

melted paraffin temp?

Answer 2

5-10°C

Question 3

cooling of paraffin embedded tissues

Answer 3

• cooled rapidly in a ref at -5°C

- immersed in cold water to solidify

Question 4

the process by which a tissue is arranged in precise positions in the mold during embedding, on the microtome before cutting and on the slide before staining

Answer 4

Orientation

Question 5

Types of blocking out molds

Answer 5

• leuckhart’s embedding mold
• compound embedding unit
• plastic embedding rings and base mold
• disposable embedding molds

Question 6

example of disposable embedding molds

Answer 6

• peel away
• plastic ice trays
• paper boats

Question 7

L-shaped (brass or metal) in metal plate w/ adjustable size

Answer 7

Leuckhart’s embedding mold

Question 8

series of interlocking plates resting on a flat metal base, forming several compartments

Answer 8

Compound embedding unit

Question 9

Stainless steel base mold fitted w/ a plastic embedding ring

Answer 9

plastic embedding rings and base mold

Question 10

plastic embedding rings and base mold is used as ______ during cutting

Answer 10

block holder

Question 11

used in tissue tek system

Answer 11

plastic embedding rings and base mold

Question 12

the process i which tissues are first infiltrated with celloidin and embedded in paraffin wax

Answer 12

double embedding method

Question 13

use for cutting large blocks of dense firm tissues (brain)

Answer 13

double embedding method

Question 14

used for undecalcified bone and for high resolution light microscopy of tissue sections thinner than the usual 4- um

Answer 14

plastic (resin) embedding

Question 15

example of tissue sections thinner than 4-6 um

Answer 15

• renal biopsies

- bone marrow biopsies

Question 16

types of plastic embedding

Answer 16

• epoxy
• polyester plastic
• acrylic plastic

Question 17

disposable thin plastic embedding molds are simply peeled off one at a time giving perfect even block without trimming

Answer 17

peel-away

Question 18

use like in ordinary refrigerators

Answer 18

plastic ice trays

Question 19

they provide easy and accurate identification of specimen, thereby avoiding confusion and interchange of tissue blocks

Answer 19

paper boats

Question 20

mixture of epoxy plastic, catalysts and accelerators

Answer 20

epoxy

Question 21

carcinogenic substance present in epoxy?

Answer 21

vinylcyclohexane dioxide (VCD)

Question 22

types of epoxy (3)

Answer 22

• bispenol A (araldite)
• glycerol (epon)
• cyclohexane (spurr)

Question 23

originally introduced for electron microscopy

Answer 23

polyester plastic

Question 24

made up of esters of acrylic or methacrylic acid that is used extensively for light microscopy

Answer 24

acrylic plastics

Question 25

type of acrylic plastics

Answer 25

polyglycol methacrylate (GMA)
methyl methacrylate (MMA)