Fresh Tissue Examination

Question 1

Four (4) methods of fresh tissue examination

Answer 1

🎉Teasing/dissociation

🎉 Squash prep/crushing

🎉 Smear prep

🎉Frozen Section

Question 2

Is a process whereby a selected tissue specimen is immersed in a watch glass containing isotonic salt solution

Answer 2

Teasing/dissociation

Question 3

A process whereby small pieces of tissue are placed in a microscopic slide and forcibly compressed with another slide or with a cover glass

Answer 3

Squash prep/crushing

Question 4

Four advantages in examining fresh tissues

Answer 4

You can observed the ff:

🎉 Motion

🎉 Mitosis

🎉 Phagocytosis

🎉 Pnocytosis

Question 5

Diameter of tissue needed in Squash prep

Answer 5

< 1mm

Question 6

(1)_______ is the process of examining sectionsor sediments, whereby cellular materials are spread lightly over a slide by means of a (2)____, (3)_____, or by making apposition smear with another (4) _____.

Answer 6

(1) Smear prep
(2) Wire loop
(3) Applicator
(4) Slide

Question 7

Smear prep is useful in (1)________, particularly for (2)______.

Answer 7

(1) Cytological examinations
(2) Cancer diagnosis

Question 8

Four (4) types of smear prep

Answer 8

(1) Streaking
(2) Spreading
(3) Pull-apart
(4) Touch prep

Question 9

A process wherein an applicator stick or a platinum loop is rapidly and gently applied in a direct or zigzag line throughout the slide

Answer 9

Streaking

Question 10

A selected portion of the material is transferred to a clean slide and gently spread into a moderately thick film

Answer 10

Spreading

Question 11

Best specimens for smear prep

Answer 11

🎉Fresh sputum

🎉 Bronchial aspirates

🎉 Thick mucoid secretions

Question 12

Best specimen for pull-apart prep

Answer 12

Smears of thick secretions such as :

🎉Serous fluids

🎉 Conc. sputum

🎉 Enzymatic lavage samples (GIT)

🎉 Blood smears

Question 13

A special method of smear preparation whereby the surface of a freshly cut piece of tissue is brought in contact and pressed on to a surface of a clean glass slide, allowing the cells to be transferred directly to the slide

Answer 13

Touch prep

Question 14

This method is normally utilized when a rapid diagnosis of the tissue in question is required

Answer 14

Frozen section

Question 15

Tissues (2) that are recommended for frozen section

Answer 15

🎉 Lipids

🎉 Nervous tissue

Question 16

Slow freezing can cause distortion of tissue due to _____.

Answer 16

Ice crystal artifacts

Question 17

4 commonly used methods of freezing

Answer 17

🎉 Liquid nitrogen

🎉 Isopentane cooled by liquid nitrogen

🎉 Carbon dioxide gas

🎉 Aerosol sprays

Question 18

Most rapid freezing agent generally used in histochemistry and during operative procedures

Answer 18

Liquid nitrogen

Question 19

Temp of liquid nitrogen that caused damaged to both block and blade

Answer 19

• 70 degrees or below

Question 20

3 freezing agents used to overcome the problems encountered in using liquid nitrogen

Answer 20

(1) Isopentane
(2) OCT
(3) Freon
- has a high thermal conductivity

Question 21

Freezing agent that is liquid at room temperature

Answer 21

Isopentane

Question 22

Temp of liquid nitrogen when small crystals start forming on the side of the beaker

Answer 22

-170 degrees celcius

Question 23

Tissues to be frozen are affixed on a (1)____, (2)_____, or (3)_______.

Answer 23

(1) Cork disc
(2) Aluminum foil
(3) Cryostat chuck

Question 24

Freezing agent taht is adequate for freezing small pieces of tissue except muscle

Answer 24

Aerosol sprays