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A. GPHCLAB > Microtomy > Flashcards

Microtomy Flashcards

1
Q

Type of microtome that is a refrigerated apparatus used in fresh tissue microtomy

A

Cryostat Microtome

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2
Q

Type of microtome mainly used for cutting celloidin embedded block

A

Sliding microtome

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3
Q

Type of microtome used for situations where rapid analysis of tissues is required

A

Cryostat microtome

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4
Q

Parts of Rotary microtome:

Part of the knife holder that adjust the knife angle

A

Knife tilt

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5
Q

Mechanical device for cutting thin uniform slices of tissue sections

A

Microtome

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6
Q

What is the thickness range of the rocking microtome?

A

2mm to 24mm

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7
Q

Most commonly used microtome used to cut thin sections of samples visible only with the aid of a light microscope

A

Rotary microtome

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8
Q

Parts of Rotary microtome:

A platform which has rails that secure the knife holder base

A

Microtome base plate or stage

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9
Q

Parts of Rotary microtome:

A part that anchors the knife holder to the microtome stage

A

Knife holder base

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10
Q

Part of rotary microtome used for section thickness

A

Micron adjustment

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11
Q

Parts of Rotary microtome:

Part of the knife holder that holds the blade

A

Blade clamp

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12
Q

Type of microtome that is used for cutting thin sections of wax embedded material of either animal or plant origin

A

Rocking microtome

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13
Q

Who invented the rotary microtome?

A

Minot

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14
Q

Cryostat microtome is used to maintain low ______ temperatures.

A

Cryogenic

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15
Q

Knives used in ultrathin microtome are usually made from _____, ______ or ________.

A

Glass
Diamond
Sapphire

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16
Q

Part of rotary microtome that turns in one direction and advances the block toward the knife at the specified microns

A

Advancement handwheel

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17
Q

Part of rotary microtome that holds the paraffin block in place

A

Casette clamp or block holder

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18
Q

Type of microtome designed to prepare very thin slices of material for study under a microscope

A

Ultrathin microtome

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19
Q

Part of sliding microtome that quickly raises or lowers the specimen 450 um per turn

A

Coarse advance/Trim control

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20
Q

Parts of Rotary microtome:

Part of the knife holder that guides the ribbons away fron the blade and towards the operator

A

Face plate

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21
Q

Part of sliding microtome that secure the guards in the chosen position

A

Black knobs

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22
Q

Means cold

A

“Cryo”

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23
Q

Type of microtome used for cutting thin to semi-thin sections of fresh. frozen tissue.

A

Freezing microtome

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24
Q

Part of sliding microtome that is moved to the central position to cover the working area of the knife

A

Knife guard

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25
Q

Rocking microtome uses what type of razor knives?

A

A-type biconcave razor knives

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26
Q

Type of microtome used in paraffin embedding block

A

Sliding microtome

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27
Q

Sliding microtome’s knife is mode ______ against a fixed block

A

Horizontally

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28
Q

Process by w/c processed tissue most commonly a paraffin embedded tissue, is trimmed and cut into uniformly thin slices or sections

A

Microtomy

29
Q

Part of rotary microtome that automatically regulates the thickness of the section

A

Ratchet feed wheel

30
Q

Cryostat microtome is maintained at a temperature between _____.

A

-5 to -30 degrees celsius (ave -20 degrees celsius)

31
Q

Type of microtome that is recommended for elementary biology and pathology students

A

Rocking microtome

33
Q

Who invented the rocking (cambridge) microtome?

A

Paldwell Trefall

34
Q

Part of rotary microtome that moves the block holder either toward the knife or away from the knife

A

Coarse handwheel

35
Q

Give four instances where the freezing microtome is especially needed.

A

Rapid diagnosis
Histological demo of fat
Neurological structures
Sensitive tissue constituents that are damaged or destroyed by heat

36
Q

what is the lenght of plane-wedge knife?

A

100 mm

37
Q

The knife of the rotary microtome is placed in a ______ position and is therefore dangerous.

A

blade-up

38
Q

Part of rotary microtome which causes reciprocal motion of the knife over the block when operated

A

Flywheel

39
Q

Basic instrument used in microtomy

A

Microtome

40
Q

Used to streched the sections under the temp. of 5 - 10 degrees celsius

A

Flotation Water bath

41
Q

Type of sliding microtome that is favored in lab where very hard tissue or large blocks are usually sectioned

A

Based-Sledge microtome

42
Q

Type of sliding microtome suited for sectioning specimens embedded in all forms of media

A

Base-sledge microtome

43
Q

Rotary microtome:

The knife and block holder are brought together by ______ and ______ motion

A

Upward, vertical

44
Q

Type of sliding microtome that are ideal for resin-embedded decalcified bone

A

Base-sledge microtome

45
Q

Type of sliding microtome wherein the block remains stationary while the knife is moved backward and forward during the process of. sectioning

A

Standard sliding microtome

46
Q

Give 3 uses of cryostat microtome in preparing thin sections of fresh tissues

A

Fluorescent Ab staining technique
Histochemical enzyme studies
Rapid preparation of urgent tissue biopsies for intraoperative diagnosis

47
Q

the ultrathin microscope is primarily used for cutting sections at _____ micra, for electron microscopy.

A

0.5 micra

48
Q

Type of sliding microtome recommended for cuttinh extremely hard and rough tissue blocks

A

Standard sliding microtome

49
Q

Sections in the base-sledge microtome are cut in a perfectly _____ plane, thereby making excellent serial tissue sections

A

Flat

50
Q

How many minutes does the cryostat freezes fresh tisssue?

A

within 2-3 mins

53
Q

The specimen used in ultrathin microtome are _____, fixed in ______, and embedded in _______.

A

Small
Osmium tetroxide
Plastic

54
Q

What is the thickness of the section in cryostat microtome?

A

4 u

55
Q

Cryostat microtome consists of a microtome, usually a ______ microtome, keep inside in a cold chamber

A

Rotary

56
Q

Type of sliding microtome used for cuttinh celloidin tissue blocks

A

Standard sliding microtome

57
Q

What is the lenght of plane-concave knife?

A

25 mm

60
Q

What is the freezing agent used in freezing microtome?

A

Liquid Carbon Dioxide

61
Q

What is the lenght of biconcave knife?

A

100 mm

62
Q

This a natural sharpening stone that removes nicks and irregularities on the knife edges

A

Hone

63
Q

What is the direction in honing?

A

Heel to toe direction

64
Q

It polish and sharpen the cutting edge

A

Stropping

65
Q

What is the direction for stropping?

A

Toe to heel

67
Q

How many strokes for honing?

A

10-20 strokes

69
Q

Type of sliding microtome consist of two movable pillars holding the adjustable knife clamps, allowing the knife to be set at an angle for cutting celloidin sections

A

Base-sledge microtome

69
Q

How many strokes for stropping?

A

40-120 strokes

70
Q

What is the bevel angle?

A

27 to 32 degrees

70
Q

A type of hone much coarse that is used for badly nicked knives

A

Fine carborundum

70
Q

It involves the removal of gross nicks on the knife edge.

A

Honing

73
Q

Type of hone that gives more polishing effect

A

Arkansas

75
Q

A type of hone used for manual sharpening when cutting edge has been rendered blunt or nicked

A

Belgium yellow

A. GPHCLAB (12 decks)

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