LQ PART I

Question 1

for cutting semi thin fresh frozen tissue which uses ____

Answer 1

freezing microtome; liquid co2

Question 2

2 L-shaped strips oh heavy brass

Answer 2

Leukhart’s embedding mold

Question 3

part of microtome for horizontal movement

Answer 3

coarse hand wheel

Question 5

type of hone which is much coarser

Answer 5

fine carborundum

Question 7

for cutting large and hard blocks of paraffin

Answer 7

sledge microtome

Question 8

direction for honing; ___strokes

Answer 8

edge first, heel to toe direction; 10-20

Question 9

3 disposable embedding mold

Answer 9

peel away
plastic ice trays
paperboat

Question 11

part of microtome for vertical movement

Answer 11

fine advance wheel

Question 12

complete penetration of by alcohol destroys infectious agents except

Answer 12

prions

Question 14

treatment for CJD

Answer 14

formalin - 48 hrs

formic acid - 1hr

Question 15

knif ised for paraffin embedded sections

Answer 15

biconcave knife

Question 17

1st and most critical step in the processing of tissue

Answer 17

fixation

Question 18

direction for stropping; ___strokes

Answer 18

toe to heel; 40-120

Question 19

temperature for cryostat; range

Answer 19

-20 C; -5 to -30 C

Question 20

clearance angle; range

Answer 20

15; 0-15

Question 21

3 essential parts of microtome

Answer 21

knife carrier and knife
block holder
ratchet feed wheel, pawl, adjustment screw

Question 22

part of tissue embedding center which holds 3 L of paraffin

Answer 22

paraffin resevoir

Question 23

An equipment that allows section to dry

Answer 23

Paraffin oven

Question 24

Paraffin oven is preffered for _____

Answer 24

Drying

Question 25

embedding method for hard tissues (bones, teeth and large sections)

Answer 25

celloidin/nitrocellulose

Question 26

6 essential parts of tissue embedding center

Answer 26

refrigerating system
paraffin melting chamber
microscreen
hot and cold orientational platforms
waste drawer
hot well

Question 27

removes gross nicks on knif edge

Answer 27

coarse honing

Question 28

causes reversible inflammatory effects

Answer 28

irritants

Question 29

health hazard which causes allergic reaction

Answer 29

sensitizers

Question 30

temperature of paraffin oven

Answer 30

2-5 C

Question 31

bevel angle

Answer 31

27-32

Question 35

water temperature (for eyes); how long

Answer 35

15-35C; 15-30 mins

Question 37

tempt of floatation water bath; fish out in ___secs

Answer 37

5-10 C;