SPORE STAIN (BARTHOLOMEW AND MITTWER COLD STAIN) Flashcards

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1
Q

What are the two genera that commonly form endospores? Presence of spores?

A

Generas:
- Bacillus & Clostridium
Yes for both

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2
Q

Under what conditions will sporulation usually occur?

A

due to harsh environmental conditions

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3
Q

What is the primary stain, decolourizer, and counterstain used in the staining of endospores?

A

Primary stain: Malachite green
Decolourizer : Water
Counterstain : Safranin

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4
Q

How does Bacillus and Clostridium can condense into an endospore? (Think about the environmental conditions)

A

when environmental conditions become too harsh to permit further vegetative growth and reproduction

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5
Q

What are the cultures used in spore stain

A

Bacillus brevis & Clostridium sporogenes

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