HOW TO USE A MICROSCOPE

Question 1

What are the two systems of lenses that is achieved from magnification of a microscope?

Answer 1

• Objective (nearest the specimen)
• Ocular (eyepiece lens/upper of the microscope)

Question 2

What is the purpose of the objective lenses? The ocular lenses?

Answer 2

Objective lenses: magnifies the specimen to produce a real image

Ocular lenses: produces the virtual image that is seen with the eye, and has a magnification of 10X

Question 3

Where are the objective lenses located at?

Answer 3

At the revolving nosepiece

Question 4

How to calculate the magnification?

Answer 4

the magnification of the ocular/eyepiece (10X) x specific power of the objective lens used (4X, 10X, 40X, 100X)

ex. ocular lens (10X) x high dry (objective)lens (40X)
= 400X

Question 5

What are the different types of objective lens magnification?

Answer 5

4X - scanning
10X - low power
40X - high dry power
100X - oil immersion (collects light at angles)

Question 6

Parts of the microscope

Answer 6

Slide holder part:
- Condenser knob
- Condenser and Iris Diaphragm

Bottom part:
- Coarse Focus Knob (big)
- Fine Focus Knob (small)

Question 7

To regulate the light:

Answer 7

• Condenser (collects and concentrate the light)
• Iris diaphragm (regulates the amount of light passing through condenser)

Question 8

Course and FIne Adjustment knobs

Answer 8

Course knob - when considerable movement is desired
Fine knob - critical focusing

Question 9

If the microscope doesn’t produce light, how would you troubleshoot it?

Answer 9

• check if the microscope is plugged in
• power is on
• iris diaphragm is open at least halfway

Question 10

If the image is foggy or dim (too bright), what might be the reason to this?

Answer 10

Foggy: lens were in contact with oil, therefore clean it with a cleaner

Dim or too bright: adjust power/intensity control, check condenser is at top