ACID FAST STAIN Flashcards

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1
Q

What is the acid fast stain?

A

a differential stain used primarily in the identification of the tuberculosis bacillus (Mycobacterium tuberculosis & Mycobacterium leprae)

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2
Q

Are the genus, Mycobacterium, stain readily by simple staining?

A

No they do not

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3
Q

Do the genus mycobacterium have waxy cell walls? Why?

A

They do have waxy cell walls, because it contains a lot of lipoidal material (mycolic acids)

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4
Q

What happens in the Ziehl-Neelsen procedure?

A

the smear is flooded with carbolfuchsin (dark red dye), then heated to penetrate the stain into the bacteria

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5
Q

What is the Kinyoun modification?

A

It is a cold stain, the concentrations of phenol and carbolfuchsin are increased and a detergent is added

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6
Q

Are all acid-fast organisms gram positive?

A

Yes, but not all gram-positive organisms are acid-fast

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7
Q

Give each an example of a acid-fast positive & negative

A

Negative : Staphylococcus epidermidis
Positive : Mycobacterium phlei

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8
Q

What is responsible for the properties of acid-fast bacteria?

A

the ability of the bacteria to resist decolourization w/ acid alcohol

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9
Q

What important genus of bacteria is used to identify in the acid fast stain?

A

Mycobacterium

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10
Q

What was the purpose of heating the stain in acid-fast technique?

Why didn’t we use Ziehl-Neelsen procedure?

A

Heat prevents the cell from being washed off the slide w/ rinsing and staining. It penetrates the cell wall

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11
Q

Do some species in the genus Mycobacterium, readily stain by simple stain or gram stain procedures?

A

No they do not

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