ISOLATION OF BACTERIAL MUTANTS Flashcards
Do bacterial mutants arise spontaneously?
Yes they do
Do bacterial mutants produce through the use of mutagenic agents (mutagens)?
YES
What are the examples of mutagens?
X-RAYS, UV Rays, nitrous acid, base analogues, and alkylating agents
T/F:
The difference depends upon how severly the genetic code was rearranged.
TRUE
What type of mutant is resistance?
This type of mutant would be resistant to inhibitory agents such as antibiotics and bacteriocins
What is the purpose of the isolation of bacterial mutant experiment?
Involves the isolation of antibiotic-resistant mutants
What are the parts of an Antibiotic Gradient plate?
TOP PART (H): nutrient agar and penicillin (bacterial suspension)
BOTTOM PART (L): low penicillin conc.
What are some examples of how bacterial mutants arise?
errors during DNA replication/radiation
