ISOLATION OF BACTERIAL MUTANTS Flashcards

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1
Q

Do bacterial mutants arise spontaneously?

A

Yes they do

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2
Q

Do bacterial mutants produce through the use of mutagenic agents (mutagens)?

A

YES

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3
Q

What are the examples of mutagens?

A

X-RAYS, UV Rays, nitrous acid, base analogues, and alkylating agents

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4
Q

T/F:
The difference depends upon how severly the genetic code was rearranged.

A

TRUE

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5
Q

What type of mutant is resistance?

A

This type of mutant would be resistant to inhibitory agents such as antibiotics and bacteriocins

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6
Q

What is the purpose of the isolation of bacterial mutant experiment?

A

Involves the isolation of antibiotic-resistant mutants

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7
Q

What are the parts of an Antibiotic Gradient plate?

A

TOP PART (H): nutrient agar and penicillin (bacterial suspension)
BOTTOM PART (L): low penicillin conc.

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8
Q

What are some examples of how bacterial mutants arise?

A

errors during DNA replication/radiation

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