pH and MICROBIAL GROWTH

Question 1

What exerts the greatest influence on an organism’s environment?

Answer 1

Hydrogen Ion Concentration

Question 2

What does the pH limits?

Answer 2

it limits the activity of enzymes with which an organism is able to synthesize new protoplasm

Question 3

What is the purpose of the pH and microbial growth experiment?

Answer 3

test the degre of inhibition of organisms that results from media containing different concentrations of hydrogen ions

Question 4

What are the four broth cultures used in the pH and microbial growth experiment?

Answer 4

Staphylococcus aureus (lvl 2)
Escherichia coli
Lactobacillus plantarum
Saccharomyces cerevisiae

Question 5

How does pH negatively affect the metabolism of microorganisms?

Answer 5

pH changes can denature enzymes by changing the charges on amino acids in proteins

Question 6

How would the pH of the culture medium be influenced by sugar fermentation?

Answer 6

By urea hydrolysis

Question 7

Is there a correlation with the bacteria used in this experiment sugar fermentation and pH

Answer 7

sugar fermentation often results in production of acids, which the pH decreases