Skin Stem Cells (15) Flashcards
What makes up skin?
a. Keratinocytes are found mostly in the dermis
b. Hair follicles grow from the epidermis
c. Pericytes are unique to the epidermis
d. Epidermis regenerates due to basal keratinocytes
d. Epidermis regenerates due to basal keratinocytes
What is not a function of skin?
a. Tough barrier against pathogens
b. Temperature control
c. Encourage water loss
d. Oil secretion
c. Encourage water loss
What is true about burns?
a. 1st degree burns involve the epidermis and dermis
b. Muscle and bone can be damaged in second degree burns
c. 3rd degree burns involve all three skin layers
d. 1st degree burns only involve the subcutaneous tissue
c. 3rd degree burns involve all three skin layers
What is not involved in culturing human skin epidermal cells?
a. Silver and iron supplements
b. An irradiated feeder layer (Swiss 3T3 J2 embryonic mouse fibroblasts)
c. Medium with cholera toxin, hydrocortisone, EGF, 10% foetal calf serum
d. Keratinocytes expand from the epidermis
a. Silver and iron supplements
What is a problem with current burn therapies?
a. Human cells are needed as feeder layers (mouse)
b. Animal products, like serum, can carry pathogens
c. Only 10% of epidermal cells from the patient grow in culture (
b. Animal products, like serum, can carry pathogens
What is a feature of CD34?
a. It is a cell surface marker on red blood cells
b. It can be found on haemopoietic stem cells and progenitor cells and hair follicle bulge region
c. Basal and super-basal bulge cells can regenerate hair follicles in the absence of CD34
d. It is unique to skin stem cells
b. It can be found on haemopoietic stem cells and progenitor cells and hair follicle bulge region
What is a feature of skin stem cells?
a. Transit amplifying cells are cycling and mostly basal
b. Early differentiation cells show intermediate long term proliferation
c. Transit amplifying cells are quiescent
d. Keratinocyte stem cells are present in large amounts
a. Transit amplifying cells are cycling and mostly basal
What is a feature of laminin10/11?
a. They repress the growth of stem cells
b. They are localised in the epithelial basement membranes
c. They can aid cell stem cell growth but do not impact tissue regeneration
d. They demonstrate that skin stem cells do not interact with the ECM
b. They are localised in the epithelial basement membranes
Which genes are expressed in the following types of stem cells?
a. Quiescent cells express epidermal differentiation markers
b. Cycling progenitor cells express Wnt inhibitors
c. Early differentiated cells express cell cycle progression genes
d. Cycling progenitor cells express genes for metabolic pathways
d. Cycling progenitor cells express genes for metabolic pathways
What is not a feature of pericytes?
a. They are localised to the epidermis
b. They can differentiates into fat, bone and cartilage
c. They Associate with basal keratinocytes and can promote epidermal cell proliferation from non-stem cells
d. They can restore adhesive structures (hemidesmosomes) and the basement membrane
a. They are localised to the epidermis
• Stem cells are responsible for organogenesis during embryonic and adult life.
T
• Third degree burns are the most painful
. F
• Cellular allografts are harvested from the patient themselves.
F
• Keratinocytes can be harvests and expanded to generate sheets and 3D cultures used to treat burns.
T
• A single haemopoietic stem cell can reconstitute the entire haemopoietic system of a mouse.
T
