Cancer Stem Cells and Epigenetics (5,10,11) Flashcards
What is not a characteristic of a malignant tumour?
a. All cells demonstrate the same, reproducible mutation
b. There are different cell populations
c. Cells can be a different state of differentiation
d. Variability is genetic and non-genetic in nature
a. All cells demonstrate the same, reproducible mutation
What best describes a theory for how tumours can develop heterogeneity?
a. The cancer stem cell theory is based on the idea that CSCs and non-CSCs can convert to one another continuously
b. The clonal evolution theory suggests that because heritable genetic and epigenetic changes are favoured in response to microenvironment pressures
c. The plastic stem cell theory suggests that Cancer cells in tumour reside in different states of stemness and differentiation
d. The cancer stem cell theory has been proved without argument
b. The clonal evolution theory suggests that because heritable genetic and epigenetic changes are favoured in response to microenvironment pressures
What is not a feature of cancer stem cells?
a. They are tumour cells with stem cell properties
b. They are resistant to chemotherapy
c. They can drive tumour growth and metastases
d. They are easily studied due to their well-known origin and marker profile
d. They are easily studied due to their well-known origin and marker profile
What would not be found in a tumour niche?
a. Vascular network
b. Secreted factors
c. Limited stromal cells
d. Extracellular matrix
c. Limited stromal cells
How do exosomes and microvesicles compare?
a. They are the same size and density, but only exosomes use exocytosis
b. Microvesicles are much larger (100-1000nm) than exosomes (30-100nm)
c. Exosomes use blebbing whilst microvesicles use exocytosis
d. Exosomes are more dense than microvesicles
b. Microvesicles are much larger (100-1000nm) than exosomes (30-100nm)
What is true about diagnosing Pancreatic Cancer using extracellular vesicles?
a. It is not yet possible to distinguish benign from precancerous
b. The test is based on the EV membrane bound proteoglycan, GPC1
c. Glypican-1 is absent in the sera of patients with pancreatic cancer
d. Invasive sampling of the pancreatic tissue is needed to look for GPC1
b. The test is based on the EV membrane bound proteoglycan, GPC1
• Tumours are heterogeneous because they have different cell populations with different mutations, different states of differentiation, different functions and genetic and non-genetic variability.
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• Cancer stem cells do not counteract the effectof drugs that specifically kill tumour cells.
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• Cancer stem cells express Oct4, Sox2 and NANOG.
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What is false about X inactivation and epigenetics?
a. A XXX female will have 3 barr bodies.
b. Changes are due to gene expression and not DNA sequence
c. Changes can be preserved through multiple cell divisions
d. A random X chromosome is inactivated in each cell of the embryo
a. A XXX female will have 3 barr bodies. (2)
What can methylation NOT lead to?
a. The recruitment of factors that modify histones
b. Nucleosomes forming tight complexes with DNA to block transcription factor binding
c. Gene activation at AT rich regions
d. The ability to differentiate a newly synthesised DNA strand from it’s template
c. Gene activation at AT rich regions
How do RNA polymerase and transcription factors access DNA?
a. When they are highly condensed as 30nm fibres
b. Following methylation of histone tails which decondenses nucleosomes
c. When the histone tails are cleaved by a transcription protease
d. Following acetylation which uncondenses nucleosomes
d. Following acetylation which uncondenses nucleosomes
What happens following the modification of histone tails?
a. The state of chromatin compaction is affected
b. Binding sites for chromatin modifying proteins are blocked
c. Polycomb protein is expressed ubiquitously
d. The methylation site is always phosphorylated
a. The state of chromatin compaction is affected
What is false about Polycomb protein?
a. It binds H3Me3K27
b. It is part of a polycomb repressor complex
c. It acts to enhance transcription
d. It maintains the repression of hox gene expression in specific developing body segments
c. It acts to enhance transcription
What is involved in the developmental consequences of DNA methylation?
a. In X chromosome inactivation, the inactive chromosome is not inherited
b. Genomic imprinting follows mendelian rules
c. Imprinting is due to differential methylation
d. Female germ cells are remethylated during meiosis in the embryonic gonad
c. Imprinting is due to differential methylation
How does insulin-like growth factor 2 demonstrate imprinting?
a. WT sperm and mutant Igf2 eggs produce small offspring
b. Mutant Igf2 sperm and WT eggs produce normal offspring
c. It is expressed from the female chromosome
d. Small offspring are produced from Igf2 mutant sperm and WT eggs
d. Small offspring are produced from Igf2 mutant sperm and WT eggs
What plays a role in considering if IPS cells have similar epigenomes to ES cells?
a. iPS cells must erase all methylation marks to revert to pluripotency
b. ES and iPS cells have exacly the same methylation patterns
c. The reversion process leads to non-random methylation events
d. Somatic memory is unique to ES cells that have been methylated
a. iPS cells must erase all methylation marks to revert to pluripotency
• Methylation of cytosines only occurs when they are followed by G.
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