Flow Cytometry and Cell Separation (24) Flashcards
What is not a physical cell separation method?
a. Hypotonic lysis
b. Immunoblotting
c. Density gradient centrifugation
d. Velocity sedimentation
b. Immunoblotting
What is enrichment?
a. How well the cells of interest are separated from the rest
b. What proportion of cells of interest in the starting population are recovered from the enriched fraction
c. The compromise between purity and recovery
d. How homogenous the separated cells of interest are
d. How homogenous the separated cells of interest are
What is involved in hypotonic lysis?
a. The process eliminates the compromise between purity and recovery
b. Separation is based on size and density
c. Resolution is time dependent
d. Cells of interest do not degrade over time
c. Resolution is time dependent
What does not impact density gradient separation?
a. The cell nucleus : cell endoplasmic reticulum ratio
b. Temperature of the gradient medium
c. Centrifugal variables like cell load
d. The viscosity and pH of the gradient medium
a. The cell nucleus : cell endoplasmic reticulum ratio
What does not influence immunomagnetic cell separation?
a. Antibody affinity
b. Bead : cell ratio
c. Antibody specificity
d. Rate of sedimentation
d. Rate of sedimentation
What does forward scatter measure?
a. Nucleus: cytoplasm ratio
b. Cell structure and complexity
c. Antibody binding affinity
d. Cell size
d. Cell size
What does side scatter measure?
a. Nucleus: cytoplasm ratio
b. Cell structure and complexity
c. Antibody binding affinity
d. Cell size
b. Cell structure and complexity
How is fluorescence detected in flow cytometry?
a. The dichroic filter selectively passes light of narrow colour range and reflects others
b. The mirror selectively passes some light while rejecting others
c. The bandpass filter reflects blue and green light
d. The laser emits red and orange light
a. The dichroic filter selectively passes light of narrow colour range and reflects others
What is NOT a strength of flow cytometry?
a. It accurately describes a cell population without the need for controls
b. Information about multiple properties of a cell can be gained simultaneously and in real time
c. It can map temporal changes in cellular parameters
d. Rare cell populations can be identified and isolated
a. It accurately describes a cell population without the need for controls
What is not a limitation of flow cytometry?
a. The cells need to be prepared as a single cell suspension
b. Resolution, enrichment and recovery are low
c. The incidence of the target cell influences the process
d. The quality of separation depends on the quality of the reagents
b. Resolution, enrichment and recovery are low
• Stem cells are the most abundant cells in bone marrow.
F
• Cells with a small nucleus : cytoplasm ratio are of low density.
T
• In velocity sedimentation, the rate of sedimentation is mostly determined by cell size.
T
• Lymphocytes demonstrate higher forward and side scatter than eosinophils.
F
• A flow cytometry reading from 2 lasers and 2 flurochromes has higher resolution than one from 1 laser and 2 flurochromes.
T
