Lung Stem Cells (26) Flashcards
Which does NOT arise during the saccular stage of lung specification?
a. Ciliated cells
b. Clara cells
c. Type I and II pneumocytes
d. Distal epithelium
d. Distal epithelium
Which best describes a growth factor involved in early lung development?
a. Fgf10 causes bud arrest
b. Bmp4 causes bud outgrowth
c. Shh causes bud branching
d. Wnt leads to bud arrest
c. Shh causes bud branching
What makes up the composition of the alveolar wall?
a. About ¼ of cells are pneumocytes
b. 90% of cells are alveolar macrophages
c. 10% of cells are endothelial
d. 5% of cells are interstitial
a. About ¼ of cells are pneumocytes
What happens when airway epithelium is exposed to cytotoxic naphthalene?
a. All cells die except for alveolar macrophages
b. CK14 positive cells take weeks to regenerate
c. Club cells are replaced and renewed within days
d. A temporal niche forms and haematopoietic stem cells are recruited
c. Club cells are replaced and renewed within days
What is not a model for slung stem cell organisation?
a. Club cells give rise to pluripotent cells by asymmetric division
b. Many unipotent progenitors give rise to stem cells
c. Stem cells are facultative and differentiated cells and de-differentiate following injury
d. There is a hierarchy of multipotent stem and progenitor cells
a. Club cells give rise to pluripotent cells by asymmetric division
What is the significance of Sca-1?
a. Cells marked with Sca-1 are incapable of forming fibroblasts
b. Sca-1 resolves lung mesenchymal progenitor cells
c. Sca-1 is specific for type II pneumocytes
d. Cells that are marked with Sca-1 cannot express EpCam
b. Sca-1 resolves lung mesenchymal progenitor cells
Lung epithelial colony forming cells are:
a. Sca-1highEpCAMpos
b. Sca-1highEpCAMneg
c. Sca-1lowEpCAMneg
d. Sca-1lowEpCAMpos
d. Sca-1lowEpCAMpos
What is the basis of the “temporal niche?”
a. Cross talk between lung epithelial and mesenchymal cells
b. The abundance of EpCAM pos cells
c. FGF and BMP signalling
d. Access to vasculature and ECM
a. Cross talk between lung epithelial and mesenchymal cells
Which is not a consideration for using lung stem cells in regenerative therapy?
a. What needs to be repaired
b. How should the treatment be delivered
c. The regenerative cells must be targeted rather then the microenvironment
d. How can treatment match the growth rate of different structural elements
c. The regenerative cells must be targeted rather then the microenvironment
• The respiratory tract contains 40-60 cell linages which are of different types and with different function.
•
T
• The lung is non-renewing
. F
• Lung CFU only differentiate to form mixed cells which have airway and alveolar association.
F
• The regenerative potential of lung stem cells is dependent on intrinsic potential of stem cell and permissive/restrictive microenvironmental cues.
T
• Influenza causes the development of Krt5+ cell clusters which migrate and restore epithelial functional.
T
The same target and same stem cells can be used when treating any disease of the lung.
F
