SERUM PROTEIN ELECTROPHORESIS AND ABNORMAL SPE PATTERNS (TOPIC 2 PROTEINS)

Question 1

Support medium for SPE

Answer 1

agarose gel
cellulose acetate

Question 2

pH of plasma proteins (ARE NEGATIVELY CHARGED)

Answer 2

8.6

Question 3

applied to immobilized the separated proteins.

Answer 3

Acetic ACid

Question 4

Dyes in SPE to visualize bands

Answer 4

Ponceau S
Amido Black
Coomassie Brilliant Blue

Question 5

SPE Stained is placed in a ?

Answer 5

densitometer

Question 6

SPE pattern
Decrease albumin
Increase in:
α1 -globulins (α1 -antitrypsin),
α2 -globulins (ceruloplasmin and haptoglobin)
β-globulin band (CRP)

Answer 6

Inflammation

Question 7

SPE pattern
Decrease in albumin.
Increase in α2 - globulin and β-globulin fractions.

Answer 7

Nephrotic syndrome

Question 8

SPE pattern
Cirrhosis
Decrease albumin
Increase γ-globulins.
Presence of β–γ bridge- β and γ regions are indistinctive.
Infectious hepatitis- γ-globulins increased
Obstructive jaundice- α2 - and β-globulins.

Answer 8

Liver disease

Question 9

SPE pattern
A spike in the γ region
Decrease albumin
β, or α2 region may also increase.

Answer 9

Monoclonal gammopathies

Question 10

SPE pattern
Decrease in Alpha 1 globin.
An inherited disorder which high risk of developing liver and lung disease.

Answer 10

Alpha-1-antitrypsin deficiency

Question 11

SPE patters INFLAMMATION

Decrease:
Increase:

Answer 11

Albumin

a1 globulin (a1 antitrypsin)
a2 globulin (ceruloplasmin, haptoglobin)
B-globin band (CRP)

Question 12

SPE patters Nephrotic Syndrome

Decrease:
Increase:

Answer 12

Albumin

a2-globulin
B-globulin fractions

Question 13

SPE patters Liver Disease

Cirrhosis
Decrease:
Increase:
Presence of?

Infectious Hepatitis
Increase:

Obstructive Jaundice:
Increase:

Answer 13

Albumin
Y-globulins
Preasence: B-y bridge

y-globulins

a2 and B-globulins

Question 14

SPE patters Monoclonal gammopathies

Decrease:
Increase:

Answer 14

Albumin

y-region
B or a2 region may also increase

Question 15

SPE patters Alpha-1-antitrypsin deficiency

Decrease:
High risk of Developing?

Answer 15

alpha-1-globin

Liver and lung disease

Question 16

(NORMAL, LOW, HIGH)
TP:
ALBUMIN:
GLOBULIN:

Cirrhosis with β-γ bridging
Hepatitis with high gamma globulins
Obstructive jaundice with high β2 macroglobulin
Acute infection: high α2-globulins
Chronic infection: high α1-globulins, α2-globulins and γ globulins
Burns and trauma

Answer 16

Normal or Low
Low
High

Question 17

(NORMAL, LOW, HIGH)
TP:
ALBUMIN:
GLOBULIN:

Nephrotic syndrome: high α2-globulins and β-globulin
Malabsorption and inadequate diet

Answer 17

Low or normal
Low
Normal

Question 18

(NORMAL, LOW, HIGH)
TP:
ALBUMIN:
GLOBULIN:

Immunodeficiency syndrome

Answer 18

Low
Normal
Low

Question 19

(NORMAL, LOW, HIGH)
TP:
ALBUMIN:
GLOBULIN:

Salt retention syndrome

Answer 19

Low
Low
Low

Question 20

(NORMAL, LOW, HIGH)
TP:
ALBUMIN:
GLOBULIN:

Dehydration

Answer 20

High
High
High

Question 21

(NORMAL, LOW, HIGH)
TP:
ALBUMIN:
GLOBULIN:

Multiple myeloma monoclonal and polyclonal gammophaties

Answer 21

High
Normal
High