AMMONIA (TOPIC 3 NON-PROTEIN)

Question 1

Produce from the deamination of amino acids during protein metabolism. Mainly by intestinal and bacterial enzymes.

Answer 1

Ammonia

Question 2

removal of amino terminal end of amino acid

Answer 2

Deamination

Question 3

most predominant in blood.

Answer 3

Ammonium

Question 4

Ammonia is converted in the liver to?

Answer 4

Urea for excretion

Question 5

Ammonia is also produce from _____ during ___

Answer 5

skeletal muscle
exercise.

Question 6

Used to asses the liver function.

Answer 6

Ammonia

Question 7

Ammonia can be quantify in ?

Answer 7

Blood and urine

Question 8

Anticoagulant for ammonia determination

Answer 8

Heparin and EDTA

Question 9

____ increases ammonia prior to test.

Answer 9

Cigarette smoking

Question 10

Ammonia determination (chemical method)

Ion-Selective Electrode (ISE)

Answer 10

Diffusion of NH3 through selective membrane and measured by potentiometer.

Question 11

Ammonia determination (chemical method)

Spectrophotometric

Answer 11

NH3 + bromphenol blue = blue color

Question 12

Ammonia determination (enzymatic method)

Glutamate dehydrogenase (GLDH) method

Answer 12

NH4 + 2-oxoglutarate + NADPH –(up arrow)GLDH,(down arrow)catalyzes)–> glutamate + NADP (340nm)

most common in automated instrument

Question 13

Normal Value for ammonia in plasma

Answer 13

19-60 ug/dl

Question 14

A rare disease of the liver mainly in children.
Deficiency in urea-cycle enzymes
Characterized by fatty liver infiltration of the liver.
Preceded by viral infection and aspirin consumption.

Answer 14

Reye’s syndrome

Question 15

impaired brain function as a result of sever liver disease. Cause by chronic hepatitis, cirrhosis or Reye’s syndrome.

Answer 15

Hepatic encephalopathy-