Seminalysis Flashcards
Which organ maintains temperature suitale for sperm production? A. vas deferens B. scrotum C. penis D. epididymis
B
Which is false?
A.Most of semen comes from prostate gland
B.Semen from seminal vesicles is gelatinous and fructose positive
C.Testes & Cowper’s gland contribute equal amount of sperm
D.NOTA
(
A - fr seminal vesicles dapat. C is true: 0.1-0.2 mL)
Which is true?
A. Epididymis is the site of maturation for sperm.
B. Prostate gland’s secreted fluid neutralizes the acidity of urine prior to semen passage.
C. Cowper’s gland’s secreted fluid initiates movement of sperm.
A
Baliktad ang B and C
Which is false?
A.A complete ejaculate liquefies in 20-25 min
B.Semen from urethra & Cowper’s gland is acidic and watery.
C.BOTA
D.NOTA
(B - this pertains to semen fr prostate
A. Describe semen from urethra & Cowper’s gland.
B. Which organ produces gelatinous semen?
A.viscous, clear
B.seminal vesicles
Enumerate 3 reasons why there is low volume of semen
Absence of seminal vesicle
Complete or partial obstruction of ejaculatory ducts
Retrograde ejaculation
A.The semen is initially in liquefied state but quickly coagulate by the action of ____
B.Proteolytic enzymes from the ___ liquefy coagulum in 20-25 minutes
A.protein kinase
B.prostate
Which is false?
A.Abnormal liquefaction may be caused by prostatic
abnormalities such as prostatitis.
B.Increased viscosity may affect sperm motility
C.Prostatic secretion & seminal vesicle
fluid are both alkaline
C - prostatic secretion is acidic
A.Semen conc is evaluated in___.
B.identify: Under the microscope, this is seen as the sperms sticking together to one another in bunches
C.in sperm motility scale,what is the normal value?
A. Mackler or Cell-VU chambers
B. sperm clumping
C. 2+
not a charcteristic of normal sperm
A. Oval form with smooth contour
B. Acrosomal cap encompassing 40-70% of head
C. No midpiece or tail abnormalities
D. With only one cytoplasmic vacuole of more than half of the sperm head
(D - dapat hindi ito present)
A. identify criteria developed in S.Africa to judge sperm motility
B. according to WHO criteria, what is considered as acceptable semen?
A. Kruger criteria
B. >30% normal forms (100 cells evaluated)
False about eval of sperm morphology
A.Higher predictive value in determining rates of pregnancy is based on the morphology of postcoital spermatozoa found at the level of the internal cervical os.
B.In normal sperm, the tail is as thin as the midpiece.
C.In normal sperm, the midpiece is axially attached.
B - slightly thinner
Which is false?
A.Not all semen samples have WBC in them
B.Leukocytes have to be differentiated from immature germ cells using immunohistochemical methods
C.Seminal plasma contains a number of antioxidants that protect sperm from oxidative damage from exposure to ROS
A - all have, pero if too much: pwedeng may infection
WBC cells → stimulate the release of reactive oxygen species (ROS) → inhibiting sperm motility and sperm function. Enumerate 3 main ROS
- Superoxide anion
- Hydrogen peroxide
- Hydroxyl radical
A. Why are viability tests used?
B. Name the 2 most common sperm viabilty tests around?
A.Viability tests are used in cases of low motility to determine the presence of live sperm versus necrozoospermia
B.Eosin test & Hypoosmotic swelling test
Which is false?
A.Eosin test- based on the fact that eosin is excluded by dead cells which are not stained
B.Hypoosmotic swelling test -The tail of only live spermatozoa is swelling in the hypoosmotic solution
C.Fructose is androgen-dependent and is produced in the seminal vesicles
A - live cells exclude eosin
Indication of either congenital absence of the vas deferens & seminal vesicles or obstruction of the ejaculatory duct are all except A. Absence of fructose B. Low semen volume C. Failure of the semen to coagulate D. Fructose excess
D
Enumerate 6 common parameters of semen evaluation
volume pH count or concentration motility morphology WBC
A.identify the seminalysis test: can identify different antibody classes involved (IgG, IgA, IgM) and location on the sperm cell (head, body or tail)
B.what category of seminalysis test does this belong to?
A.Immunobead Rosette Test
B.antisperm antibodies test
A.purpose of computer-assisted semen analysis
B.T/F : Absence of acrosome reaction implies poor prognosis for fertilization
A.Mostly for assessment of sperm concentration and specific patterns of sperm motility (velocity, linearity etc)
B.T
Enumerate 2 purposes of Hamster Egg Penetration Assay
- to ensure the fertilizing capability of each donor candidate’s sperm
- To check sperm fusion ability
A.identify the diagnostic test:Two halves of human zona pellucida is incubated with patient’s capacitated sperm and control fertile donor’s sperm
B.main purpose of A :)
A.hemizona test
B.To evaluate sperm zona-binding capacity
Retrograde ejaculation is usually seen in the ff patients, except:
A. With diabetes
B. After transurethral surgery
C. With hearing problems
D. After retroperitoneal lymph nodes dissection
E. With spinal cord injuries
C
Poor sperm tests can result from 4 main reasons. Enumerate!
- Incorrect semen collection technique
- Too long time delay bet collection & testing
- Too short interval since the previous ejaculation
- Recent systemic illness in the last 3 months
Donor semen candidates are required to complete 3 things. Enumerate!
- Three-generation medical, genetic, and social history
- Personal interview
- Physical examination with a physician
At post-thaw analysis, three criteria must be met by semen to be considered acceptable. enumerate
- 20 million motile cells/mL,
- 25% motility, and
- 50% cryosurvival
A. Jewish semen donors are tested for:
B. Mediterranean semen donors are tested for:
C. Afro-American/African semen donors are tested for:
A. Tay-Sachs
B. Thalassemia
C. Sickle cell anemia
