Cytology Flashcards
Which is false?
A.Not all tumors exfoliate cells.
B.Scrapings are more difficult to collect and only able to collect superficial lesions.
C.The material must be allowed to dry on slide before performing squash prep or other smear technique.
D.NOTA
C - must not be allowed dapat
A.Differentiate scrapings from imprints
B.Main advantages of fine needle aspirates
A. Scrapings are done on freshly cut surfaces; imprints are done on ulcers/external lesions/biopsy
B. Avoids superficial contamination & Very little risk to patient
A.Fine Needle Aspirate Non-Aspiration Technique works best __ & for __
B.needle used @ FNA Aspiration??
A.for small masses that are difficult to aspirate & or highly vascular tissues
B.22-25 gauge needle
A.Purpose of fixation
B.best fixative
A.Prevents cellular distortion
B.50% ethyl alcohol is the best fixative
A.Why shouldn’t the solution be >50% EtOH ?
B. Why shouldn’t we use fixatives containing acetones or ether for liquid specimens?
A. hardens sediments, very difficult to spread on slides
B. cannot be smeared immediately after collection
Enumerate three ways to prepare smears from aspirates?
- Squash Preparation method
- Needle Spread method
- Blood Smear method
In which method is the sample pulled out into several projections (starfish appearance)?
A.Squash Preparation method
B.Needle Spread method
C.Blood Smear method
(B! A is when Aspirated material is placed on the center of the slide. A second slide is placed over the sample to form a cross.)
One of the common problems with Fine Needle Aspirate is the minimal number of cells obtained. Which is not a probable reason?
A.Some lesions do not exfoliate cells well.
B.The needle may miss the site of the lesion
C.Timid collection
D.Inadequate positive pressure
D - negative pressure dapat
One of the common problems with Fine Needle Aspirate is blood contamination. Which is not a probable reason?
A.Using too small needle gauge
B.Prolonged aspiration
C.Failure to blot if doing imprint
A: too large dapat
False about coating fixatives
A.spray form
B.for smears which to be mailed to other laboratories
C.has to be removed by 80% isopropyl alcohol before staining
C = ethyl alcohol dapat
A.Fixative best used for smears prepared from fluids?
B.stain used in human OB-Gyne exams?
A.95% ethyl alcohol
B.Papanicolau stain
False about 95% ethyl alcohol
A.Do not excessively shrink or swell cells.
B.Do not distort or dissolve cellular components.
C.Preserves nuclear details without inactivating enzymes.
D.Improves optical differentiation and enhance staining properties of the cell components.
C: enzymes are also activated!
False about Papanicolau stain
A. highly define nuclear details (differentiates benign from malignant)
B. cytoplasm is clearly stained
C. various shades of one tint delineates degrees of cellular maturity and metabolic activity
C - various hues dapat
[Papanicolau Staining]
- Rgt for nuclear staining?
- Rgt for cytoplasmic staining?
- 90-96% ethyl alcohol
2. OG-6 and EA-50
[Papanicolau Staining]
- Rgt for dehydration?
- Rgt for clearing?
- ethanol
2. xylene