SECTION CUTTING Flashcards
Process whereby tissues are cut into uniformly thin slices or “sections” with the aid of a microtome, to facilitate microscopic study
CUTTING OF SECTIONS
Where the tissue is held in position
Block holder
For actual cutting of tissue sections
Knife carrier and Knife
To line up the tissue block in proper position with the knife, for adjusting the proper thickness of the tissue
Pawl, Ratchet feed wheel and Adjustment Screws
Simplest type. Serial sections of small and large blocks of paraffin embedded tissues
Rocking/Cambridge
Who introduced Rocking/Cambridge
Paldwell-Trefall
Section thickness using Cambridge microtome
10-12 µ thick
COMMONLY USED FOR ROUTINE AND RESEARCH LABORATORIES
Rotary/Minot
Heavier and more stable than Rocking Microtome
Rotary/Minot
MOST COMMONLY USED FOR ROUTINE PARAFFIN EMBEDDED SECTIONS
Rotary/Minot
Who introduced Rotary/Minot
Minot
Section thickness using Minot microtome
4-6 µ thick
For celloidin embedded sections
Sliding microtome
MOST DANGEROUS TYPE OF MICROTOME
Sliding microtome
Has 2 movable pillars that holds the adjustable knife clamps
Base Sledge Sliding
Who introduced Sliding microtome
Adams
Section thickness using Sliding microtome
7-9 µ thick
Unembedded frozen sections
Freezing microtome
Who introduced Freezing microtome
Queckette
Section thickness using Freezing microtome
10-15 µ thick
Electron microscopy
Ultrathin Sectioning
Section thickness using Ultrathin Sectioning
0.5 µ thick
Apparatus capable of freezing the tissue into the blockholder to the correct degree of hardness to facilitate easier and faster sectioning
Cold/Cryostat Microtome
Fluorescent Ab Staining; Fresh Tissue Examination; Urgent Biopsies
Cold/Cryostat Microtome
Cold/Cryostat environment is kept at what temperature
-5 to -30°C (average -20°C)
Cold/Cryostat Microtome is capable of freezing fresh tissues within
2-3 minutes
Paraffin sections are cut between
4-6 µ thick for routine procedures
Knife is usually tilted at ____ angulation on a microtome to allow a clearance angle between the cutting facet and the tissue block
0-15°
Temperature of water bath
6-10°C lower than the melting point of the wax used for embedding the tissue
The process of lifting out tissue sections from water bath
FLOATING-OUT or FISHING-OUT
The purpose of floating out bath for tissue ribbon is
To flatten folds and creases
Tissue sections are then placed in a paraffin oven (maintained at a temperature _____ the melting point of paraffin wax) to dry
2-5°C above
Celloidin embedded tissue blocks which may be cut by sliding microtome, sections are cut between
7-9µ thick/ >8µ
May be cut from fresh or fixed tissues frozen with CO2, and to cut in a freezing microtome or Cryostat
FROZEN SECTIONS
