EMBEDDING/CASTING/BLOCKING Flashcards
Process by which the impregnated tissue is placed into a precisely arranged position in a mold containing a medium which is then allowed to solidify
EMBEDDING
Process by which a tissue is arranged in precise positions in the mold during embedding, on the microtome before cutting, and on the slide before staining
ORIENTATION
Blocking-out Molds
Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Rings and Base molds
2 L-shaped strips of heavy brass or metal arranged on a flat metal plate
Leuckhart’s Embedding Mold
Routinely used embedding molds
Leuckhart’s Embedding Mold
Series of interlocking plates resting on a flat metal base, forming several compartments
Compound Embedding Unit
Special stainless steel base mold fitted with a plastic embedding ring which later serves as the block holder during cutting
Plastic Embedding Rings and Base molds
Disposable Embedding Molds
Peel-Away
Plastic Ice Trays
Paper Boats
Disposable thin plastic embedding molds which are simply peeled off as soon as the wax has solidified
Peel-Away
Mold used in busy laboratories
Plastic Ice Trays
Cheap, easy to make, provide accurate identification of specimen, can be made to suit any size of tissue
Paper Boats
Embedding molds should bear the case number, other identification data of the tissue block within. True or False?
True
Recommended for embedding hard tissues (e.g., bones, teeth), and for large sections of whole organs (e.g., eye)
Celloidin or Nitrocellulose Method
Tissues are first infiltrated with celloidin and subsequently embedded in paraffin; used to facilitate cutting of large blocks of dense firm tissues (e.g., brain), and for making small sections of celloidin blocks
Double-Embedding Method
