CLEARING/DEALCOHOLIZATION

Question 1

Process whereby alcohol is removed from the tissue and replaced with a substance that will dissolve the wax with which the tissue is to be impregnated

Answer 1

CLEARING

Question 2

Commonly used clearing agents:

Answer 2

Xylene, toluene, benzene, chloroform, cedarwood oil, aniline oil, clove oil and CCI4

Question 3

Clearing agents should be miscible with alcohol, paraffin and/or mounting medium. True or False?

Answer 3

True

Question 4

Urgent biopsies; carcinogenic: bone marrow damage and aplastic anemia

Answer 4

Benzene

Question 5

Tissue:Clearing agent ratio

Answer 5

1:10

Question 6

MOST COMMON; embedding and mounting procedures

Answer 6

Xylene (Xylol)

Question 7

Clearing time of Xylene

Answer 7

15mins-1hour

Question 8

Routine post-mortem research tissues

Answer 8

Toluene

Question 9

Clearing time of Toluene

Answer 9

1-2hours

Question 10

Urgent biopsies; carcinogenic: bone marrow damage and aplastic anemia

Answer 10

Benzene

Question 11

Clearing time of Benzene

Answer 11

15mins-1hour

Question 12

CNS tissues, smooth muscles, skin, and cytological studies

Answer 12

Cedar Wood Oil

Question 13

Clearing time of Cedar Wood Oil

Answer 13

Paraffin sections: 2-3 days
Celloidin sections: 5 -6 days

Question 14

Lymph nodes, embryo, nervous tissue

Answer 14

Chloroform

Question 15

Clearing time of Chloroform

Answer 15

6-24hours

Question 16

Embryos, insects, and very delicate specimens

Answer 16

Aniline Oil

Question 17

Slow, minimum shrinkage tissues, adultered tissue

Answer 17

Clove Oil

Question 18

For embedding process; similar to chloroform

Answer 18

Carbon Tetrachloride

Question 19

Dehydrating and clearing agent

Answer 19

Tetrahydrofuran and Dioxane

Question 20

Substitute of Cedar wood oil

Answer 20

Terpinol

Question 21

Large pieces of tissues and embryonic materials

Answer 21

Amyl acetate