DEHYDRATION Flashcards
Process of removing intracellular and extracellular water from the tissue following fixation and prior to wax impregnation, by means of DEHYDRATING AGENTS
DEHYDRATION
Tissue:Dehydrant ratio
1:10
MOST COMMONLY USED
Ethyl alcohol
Routine procedures, BEST DEHYDRATING AGENT; fast acting, mixes with water, penetrates easily, not poisonous, not expensive
Ethyl alcohol
Blood and tissue films, smear preparation, toxic
Methyl alcohol
Plant and animal microtechniques, slow action
Butyl alcohol
Cheap, rapid-acting, used for urgent biopsies
Acetone
Expensive, highly toxic vapor, not routinely used
Dioxane (diethylene dioxide)
Dehydrates rapidly, not harmful to the tissues
Cellosolve
Other name for Cellosolve
Ethylene glycol monoethyl ether
Dehydrates rapidly, produces little distortion and hardening of tissues
Triethyl phosphate
Dehydrating and clearing agent
Tetrahydrofuran